caa a22 4500 445852550 CHVBK 20180317145417.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00204-010-0543-7 doi (NATIONALLICENCE)springer-10.1007/s00204-010-0543-7 Perinatal cisplatin exposure induces cochlear apoptosis in newborn guinea pigs [Elektronische Daten] [Shuai Hao, Xuejun Jiang, Aihui Yan, Bo Yang] The objective of this study was to evaluate the role of apoptosis in the development of the newborn cochlear structures and hearing loss caused by prenatal cis-diaminedichloroplatinum (cisplatin) exposure. Pregnant albino guinea pigs were intraperitoneally injected with 1.5mg/kg body weight cisplatin once a day for seven consecutive days at gestational day (GD) 51 to GD 57. At postnatal day (PND) 14, pups were examined in the distortion product otoacoustic emission (DPOAE) task. The temporal bones were then removed and immunohistochemically stained for caspase 3, using the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) method. Cisplatin used during pregnancy could induce hearing loss in newborn and cochlear hair cell apoptosis. In conclusion, apoptosis may play an important role in the development of hearing impairment, caused by perinatal cisplatin exposure. Springer-Verlag, 2010 Cis -diaminedichloroplatinum (cisplatin) nationallicence Perinatal exposure nationallicence Cochlear apoptosis nationallicence Caspase-3 nationallicence Newborn nationallicence Hao Shuai Department of Otolaryngology, First Affiliated Hospital of China Medical University, No. 155, Nanjing North Street, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut Jiang Xuejun Department of Otolaryngology, First Affiliated Hospital of China Medical University, No. 155, Nanjing North Street, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut Yan Aihui Department of Otolaryngology, First Affiliated Hospital of China Medical University, No. 155, Nanjing North Street, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut Yang Bo Department of Otolaryngology, First Affiliated Hospital of China Medical University, No. 155, Nanjing North Street, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut Archives of Toxicology Springer-Verlag 85/1(2011-01-01), 19-25 0340-5761 85:1<19 2011 85 204 https://doi.org/10.1007/s00204-010-0543-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0543-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hao Shuai Department of Otolaryngology, First Affiliated Hospital of China Medical University, No. 155, Nanjing North Street, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Jiang Xuejun Department of Otolaryngology, First Affiliated Hospital of China Medical University, No. 155, Nanjing North Street, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Yan Aihui Department of Otolaryngology, First Affiliated Hospital of China Medical University, No. 155, Nanjing North Street, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Yang Bo Department of Otolaryngology, First Affiliated Hospital of China Medical University, No. 155, Nanjing North Street, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/1(2011-01-01), 19-25 0340-5761 85:1<19 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer