caa a22 4500 445852569 CHVBK 20180317145417.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00204-010-0523-y doi (NATIONALLICENCE)springer-10.1007/s00204-010-0523-y Preservation of uroplakins by 2-mercaptoethanesulfonate in cyclophosphamide-induced rat cystitis [Elektronische Daten] [Yoon Kyung, Hee Park, Gilho Lee] Cyclophosphamide (CP) causes extensive cystitis, which is ameliorated with concomitant treatment with mesna. We investigated the protective mechanisms of mesna in the expression of uroplakin (UP), a strong mucosal barrier against toxic materials, in CP-induced rat cystitis. A total of 54 SD female rats received a single intraperitoneal injection of 200mg of CP/kg. Six CP-treated, 6 CP+mesna (120mg/kg)-treated rats, and 6 negative controls were sequentially sacrificed at 12, 24, and 72h post-CP injection. The bladders were harvested. The levels of UPIa, Ib, II, and III mRNA on real-time PCR, the UPII and III expressions on immunoblotting, and the UPII expression on immunolocalization study in the harvested bladder were maximally suppressed within 12-24h, whereas partially or completely recovered at 24-72h post-CP injection. In addition, the responses in UPs after a CP insult were heterogeneous (i.e., markedly suppressed in UPII and lesser destructive in UPIII). Even though the mesna-treated rats also showed transient and small reductions in the mRNA levels of all UPs, mesna clearly preserved the UP expressions of mRNA and protein in CP-induced urinary bladder mucosa. In conclusion, this study suggests that CP transiently reduces the expression of UPs and mesna protects the urinary bladder mucosa through the preservation of UPs protein. Springer-Verlag, 2010 Cyclophosphamide nationallicence Mesna nationallicence Cystitis nationallicence Uroplakin nationallicence Rat nationallicence Kyung Yoon Department of Urology, Dankook University College of Medicine, San16-5, Ansedong Cheonan Si, 330-715, Chungcheoung Nam Do, South Korea aut Park Hee Department of Urology, Dankook University College of Medicine, San16-5, Ansedong Cheonan Si, 330-715, Chungcheoung Nam Do, South Korea aut Lee Gilho Department of Urology, Dankook University College of Medicine, San16-5, Ansedong Cheonan Si, 330-715, Chungcheoung Nam Do, South Korea aut Archives of Toxicology Springer-Verlag 85/1(2011-01-01), 51-57 0340-5761 85:1<51 2011 85 204 https://doi.org/10.1007/s00204-010-0523-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0523-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kyung Yoon Department of Urology, Dankook University College of Medicine, San16-5, Ansedong Cheonan Si, 330-715, Chungcheoung Nam Do, South Korea aut NATIONALLICENCE 700 1- Park Hee Department of Urology, Dankook University College of Medicine, San16-5, Ansedong Cheonan Si, 330-715, Chungcheoung Nam Do, South Korea aut NATIONALLICENCE 700 1- Lee Gilho Department of Urology, Dankook University College of Medicine, San16-5, Ansedong Cheonan Si, 330-715, Chungcheoung Nam Do, South Korea aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/1(2011-01-01), 51-57 0340-5761 85:1<51 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer