caa a22 4500 44585264X CHVBK 20180317145418.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00204-010-0537-5 doi (NATIONALLICENCE)springer-10.1007/s00204-010-0537-5 Proteomic analysis of brain proteins of rats exposed to high fluoride and low iodine [Elektronische Daten] [Yaming Ge, Ruiyan Niu, Jianhai Zhang, Jundong Wang] Epidemiological investigations reveal that high fluoride and low iodine have strong adverse effects on the intelligence quotient (IQ) of children. Studies also report that in some high fluoride areas, iodine deficiency also exists, especially in China. Here, with the proteomic techniques, we first report on the proteomic changes in brain proteins in offspring rats at postnatal day 20 exposed to high fluoride and/or low iodine. To investigate molecular mechanisms of central neural system injury induced by the above two elements, proteins were isolated and profiled by two-dimensional gel electrophoresis (2DE). By the analysis of Image-Master 2D Elite software, 71 protein spots in 2DE gels of treatment groups were gained and up- or down-regulated by two folds, and 5 proteins were regulated by five folds, with the comparison to the control group. The proteins changed by five folds were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The identified proteins are mainly related with cellular signaling, energy metabolism, and protein metabolism and provide a valuable clue to explore the mechanism underlining the neurotoxicity of high fluoride and low iodine. Moreover, these results could provide potential biomarkers for hazards caused by excessive fluoride and low iodine. Springer-Verlag, 2010 Brain protein nationallicence Fluoride nationallicence Iodine deficiency nationallicence Two-dimensional electrophoresis nationallicence Mass spectrometry nationallicence Proteomic nationallicence Ge Yaming Shanxi Key Lab of Environ-Veterinary Medicine, Shanxi Agricultural University, 030801, Taigu, Shanxi, People's Republic of China aut Niu Ruiyan Shanxi Key Lab of Environ-Veterinary Medicine, Shanxi Agricultural University, 030801, Taigu, Shanxi, People's Republic of China aut Zhang Jianhai Shanxi Key Lab of Environ-Veterinary Medicine, Shanxi Agricultural University, 030801, Taigu, Shanxi, People's Republic of China aut Wang Jundong Shanxi Key Lab of Environ-Veterinary Medicine, Shanxi Agricultural University, 030801, Taigu, Shanxi, People's Republic of China aut Archives of Toxicology Springer-Verlag 85/1(2011-01-01), 27-33 0340-5761 85:1<27 2011 85 204 https://doi.org/10.1007/s00204-010-0537-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0537-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ge Yaming Shanxi Key Lab of Environ-Veterinary Medicine, Shanxi Agricultural University, 030801, Taigu, Shanxi, People's Republic of China aut NATIONALLICENCE 700 1- Niu Ruiyan Shanxi Key Lab of Environ-Veterinary Medicine, Shanxi Agricultural University, 030801, Taigu, Shanxi, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Jianhai Shanxi Key Lab of Environ-Veterinary Medicine, Shanxi Agricultural University, 030801, Taigu, Shanxi, People's Republic of China aut NATIONALLICENCE 700 1- Wang Jundong Shanxi Key Lab of Environ-Veterinary Medicine, Shanxi Agricultural University, 030801, Taigu, Shanxi, People's Republic of China aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/1(2011-01-01), 27-33 0340-5761 85:1<27 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer