caa a22 4500 445852666 CHVBK 20180317145418.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00204-011-0706-1 doi (NATIONALLICENCE)springer-10.1007/s00204-011-0706-1 Simple and quick method for whole-liver decellularization: a novel in vitro three-dimensional bioengineering tool? [Elektronische Daten] [Joery De Kock, Liesbeth Ceelen, Ward De Spiegelaere, Christophe Casteleyn, Paul Claes, Tamara Vanhaecke, Vera Rogiers] Proof of principle of organ reengineering through the development of a transplantable recellularized liver graft was published recently. As the decellularization time of the rat liver took 72h, loss of some key matrix proteins seemed inevitable. Here, we describe the development of a three-dimensional naturally derived liver scaffold with an intact microvascular system that is capable of withstanding fluid flows in the three hepatic circular systems and that is obtained within 60min. For this purpose, whole rat livers were sequentially perfused with a selection of mild tensioactive substances to remove the cellular components while preserving the major extracellular matrix proteins, including laminin, collagen I, collagen IV, and fibronectin. In addition, we could show the presence of extracellular matrix-bound growth factor islets, important for cell engraftment, migration, proliferation, and differentiation. This easy to prepare scaffold could represent a remarkable tool in the bioengineering of complex three-dimensional in vitro systems for advanced preclinical drug development. Springer-Verlag, 2011 Liver matrix nationallicence Organ decellularization nationallicence Biological liver scaffold nationallicence Three-dimensional scaffold nationallicence DLM : Decellularized liver matrix nationallicence ECM : Extracellular matrix nationallicence KHB : Krebs-Henseleit buffer nationallicence PBS : Phosphate-buffered saline nationallicence SEM : Scanning electron microscopy nationallicence SDS : Sodium dodecyl sulfate nationallicence 3D : Three-dimensional nationallicence VEGF : Vascular endothelial growth factor nationallicence De Kock Joery Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium aut Ceelen Liesbeth Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium aut De Spiegelaere Ward Department of Morphology, Faculty of Veterinary Medicine, Universiteit Gent (UGent), Salisburylaan 133, 9820, Merelbeke, Belgium aut Casteleyn Christophe Department of Morphology, Faculty of Veterinary Medicine, Universiteit Gent (UGent), Salisburylaan 133, 9820, Merelbeke, Belgium aut Claes Paul Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium aut Vanhaecke Tamara Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium aut Rogiers Vera Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium aut Archives of Toxicology Springer-Verlag 85/6(2011-06-01), 607-612 0340-5761 85:6<607 2011 85 204 https://doi.org/10.1007/s00204-011-0706-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-011-0706-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- De Kock Joery Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium aut NATIONALLICENCE 700 1- Ceelen Liesbeth Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium aut NATIONALLICENCE 700 1- De Spiegelaere Ward Department of Morphology, Faculty of Veterinary Medicine, Universiteit Gent (UGent), Salisburylaan 133, 9820, Merelbeke, Belgium aut NATIONALLICENCE 700 1- Casteleyn Christophe Department of Morphology, Faculty of Veterinary Medicine, Universiteit Gent (UGent), Salisburylaan 133, 9820, Merelbeke, Belgium aut NATIONALLICENCE 700 1- Claes Paul Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium aut NATIONALLICENCE 700 1- Vanhaecke Tamara Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium aut NATIONALLICENCE 700 1- Rogiers Vera Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, 1090, Brussels, Belgium aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/6(2011-06-01), 607-612 0340-5761 85:6<607 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer