caa a22 4500 445852674 CHVBK 20180317145418.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00204-010-0606-9 doi (NATIONALLICENCE)springer-10.1007/s00204-010-0606-9 In vitro aneugenic effects of the fungicide thiabendazole evaluated in human lymphocytes by the micronucleus assay [Elektronische Daten] [Alfredo Santovito, Piero Cervella, Massimiliano Delpero] Thiabendazole is a benzimidazole-derived compound widely employed in agriculture as anthelmintic and fungicide. It is also used as a post-harvest fungicide for imported citrus fruits during transport and storage, and thus, it was found at high concentration in fruits and vegetables. Several studies have analyzed the potential genotoxic effect of thiabendazole on different prokaryotic and eukaryotic systems, but in many cases, results were contradictory. In the present study, the genotoxic potential of thiabendazole have been evaluated, by micronucleus assay in freshly isolated human peripheral lymphocytes. The cells were incubated with 0.5, 5 and 50μg/ml concentrations of the tested substance for 48h at 37°C. Mitomycin C at final concentration of 0.01μg/ml culture was used as a positive control. The results indicated that the thiabendazole significantly (P<0.05) increased the micronucleus frequency compared with the negative control in all treatment concentrations, indicating a potential aneugenic hazard of thiabendazole in cultured human peripheral lymphocytes. The cytokinesis-block proliferation index value, however, was not decreased significantly compared with the negative control. Significant (P<0.05) differences in the micronuclei frequency were also found between the lower dose (0.5μg/ml) and the other two analyzed doses of thiabendazole. In contrast, no differences were found between 5 and 50μg/ml of thiabendazole and between DMSO and negative control. Finally, control cultures treated with the known mutagen MMC showed a very consistent increase in MN with respect to the negative controls. Springer-Verlag, 2010 Pesticide nationallicence Genotoxicity nationallicence Cytogenetic study nationallicence Benzimidazole nationallicence Santovito Alfredo Dipartimento di Biologia Animale e dell'Uomo, Università di Torino, Via Accademia Albertina 13, 10123, Turin, Italy aut Cervella Piero Dipartimento di Biologia Animale e dell'Uomo, Università di Torino, Via Accademia Albertina 13, 10123, Turin, Italy aut Delpero Massimiliano Dipartimento di Biologia Animale e dell'Uomo, Università di Torino, Via Accademia Albertina 13, 10123, Turin, Italy aut Archives of Toxicology Springer-Verlag 85/6(2011-06-01), 689-693 0340-5761 85:6<689 2011 85 204 https://doi.org/10.1007/s00204-010-0606-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0606-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Santovito Alfredo Dipartimento di Biologia Animale e dell'Uomo, Università di Torino, Via Accademia Albertina 13, 10123, Turin, Italy aut NATIONALLICENCE 700 1- Cervella Piero Dipartimento di Biologia Animale e dell'Uomo, Università di Torino, Via Accademia Albertina 13, 10123, Turin, Italy aut NATIONALLICENCE 700 1- Delpero Massimiliano Dipartimento di Biologia Animale e dell'Uomo, Università di Torino, Via Accademia Albertina 13, 10123, Turin, Italy aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/6(2011-06-01), 689-693 0340-5761 85:6<689 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer