caa a22 4500 445852747 CHVBK 20180317145418.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00204-010-0604-y doi (NATIONALLICENCE)springer-10.1007/s00204-010-0604-y PARP-1 expression and activity in primary human lung cells [Elektronische Daten] [Mohamed Ahmad, Abdelrahman Torky, Felix Glahn, Robert Scheubel, Heidi Foth] Activation of poly(ADP-ribose) polymerase-1 (PARP-1) in response to DNA damage is an important mechanism to keep homeostasis or to trigger apoptosis. The expression and function of (PARP-1) was studied in primary cells cultured from human lung. Normal human bronchial epithelial cells (NHBEC) and peripheral lung cells (PLC) from lung cancer patients were grown as explant cultures and were followed over a period of 12weeks. PARP-1 protein was expressed in all explant cultures from bronchial epithelium. The levels of PARP protein differed between individuals by a factor of 2.3 in the first explant. Three cases were followed for more than 100days. The expression levels varied intra-individually by a factor of 1.3-1.4 over this time period. PARP-1 activity was determined immunohistochemically after induction of DNA damage with H2O2 (0.05-0.3mM, 5min). The fluorescence signal for ADP-ribose polymers attached to chromatin proteins correlated well with the concentration of H2O2. PARP-1 activity differed by a factor of 3.1 in NHBECs obtained from the first generation of explants from 11 cases. PARP-1 activity is present in NHBECs until the 8th and in PLCs until the 12th week and declined to about half of the start level. Primary cultures of NHBECs and PLC are suitable to study the effect of external factors on PARP-1 expression and function. Springer-Verlag, 2010 NHBEC nationallicence PLC nationallicence Long-term cultivation nationallicence PARP-1 protein expression nationallicence PARP-1 activity nationallicence Inter-individual variation nationallicence Ahmad Mohamed Institute of Environmental Toxicology, Martin Luther University Halle Wittenberg, Franzosenweg 1a, 06097, Halle, Saale, Germany aut Torky Abdelrahman Institute of Environmental Toxicology, Martin Luther University Halle Wittenberg, Franzosenweg 1a, 06097, Halle, Saale, Germany aut Glahn Felix Institute of Environmental Toxicology, Martin Luther University Halle Wittenberg, Franzosenweg 1a, 06097, Halle, Saale, Germany aut Scheubel Robert Department of Cardiothoracic Surgery, Martin-Luther University Halle Wittenberg, 06120, Halle, Germany aut Foth Heidi Institute of Environmental Toxicology, Martin Luther University Halle Wittenberg, Franzosenweg 1a, 06097, Halle, Saale, Germany aut Archives of Toxicology Springer-Verlag 85/6(2011-06-01), 669-679 0340-5761 85:6<669 2011 85 204 https://doi.org/10.1007/s00204-010-0604-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0604-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ahmad Mohamed Institute of Environmental Toxicology, Martin Luther University Halle Wittenberg, Franzosenweg 1a, 06097, Halle, Saale, Germany aut NATIONALLICENCE 700 1- Torky Abdelrahman Institute of Environmental Toxicology, Martin Luther University Halle Wittenberg, Franzosenweg 1a, 06097, Halle, Saale, Germany aut NATIONALLICENCE 700 1- Glahn Felix Institute of Environmental Toxicology, Martin Luther University Halle Wittenberg, Franzosenweg 1a, 06097, Halle, Saale, Germany aut NATIONALLICENCE 700 1- Scheubel Robert Department of Cardiothoracic Surgery, Martin-Luther University Halle Wittenberg, 06120, Halle, Germany aut NATIONALLICENCE 700 1- Foth Heidi Institute of Environmental Toxicology, Martin Luther University Halle Wittenberg, Franzosenweg 1a, 06097, Halle, Saale, Germany aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/6(2011-06-01), 669-679 0340-5761 85:6<669 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer