caa a22 4500 445852771 CHVBK 20180317145418.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00204-010-0603-z doi (NATIONALLICENCE)springer-10.1007/s00204-010-0603-z Production of liver preneoplasia and gallbladder agenesis in turkey fetuses administered diethylnitrosamine [Elektronische Daten] [Gary Williams, Klaus Brunnemann, Michael Iatropoulos, Daniel Smart, Harald Enzmann] The in ovo carcinogenicity assessing (IOCA) assay was used to examine the morphological changes in fetal turkey livers caused by the DNA-reactive carcinogen diethylnitrosamine (DEN). Fertilized turkey eggs were allocated into 3 groups: nondosed control (NDC), vehicle (water) control (VC) and DEN-dosed. At day 0, the fertilized eggs of the dosed groups were injected with 1 (LD) or 4 (HD) mg/egg (about 12.5 or 50 mg/kg egg) of DEN and the VC were injected with water. All eggs were allowed to incubate at 37°C and 60% humidity for 24days. The fetal livers were collected and processed for histopathological evaluation (H&E staining). Typical survival rates were 82% for the NDC, 50% for the VC and 16-65% for the DEN-dosed fetuses. No difference in histology was found between NDC and VC control groups. Both DEN concentrations produced dose-related liver findings consisting of foci of altered hepatocytes (FAH), which had assumed a tubular cord (glandular) pattern, and in HD DEN group the FAH assumed a tumor-like morphology. In addition, the high DEN dose produced gallbladder agenesis. Thus, DEN produced both hepatocellular transformation (FAH) similar to preneoplastic microscopic changes in adult rodents, reflecting disruption of the fetal processes of differentiation and proliferation, and also teratogenicity (gallbladder agenesis). Springer-Verlag, 2010 In ovo carcinogenicity assessing assay in turkey nationallicence Diethylnitrosamine nationallicence Fetal liver differentiation nationallicence Proliferation and preneoplasia nationallicence Gallbladder agenesis nationallicence BfArM : Federal Institute for Drugs and Medical Devices of Germany nationallicence DEN : Diethylnitrosamine nationallicence FAH : Foci of altered hepatocytes nationallicence GLP : Good Laboratory Practices for Nonclinical Laboratories nationallicence HD : High dose of DEN nationallicence H&E : Hematoxylin and eosin stain nationallicence IOCA : In ovo carcinogenicity assessing assay nationallicence LD : Low dose of DEN nationallicence NDC : Nondosed control nationallicence NOAEL : No adverse effect level nationallicence VC : Vehicle (water) control nationallicence Williams Gary New York Medical College, 10595, Valhalla, NY, USA aut Brunnemann Klaus New York Medical College, 10595, Valhalla, NY, USA aut Iatropoulos Michael New York Medical College, 10595, Valhalla, NY, USA aut Smart Daniel New York Medical College, 10595, Valhalla, NY, USA aut Enzmann Harald New York Medical College, 10595, Valhalla, NY, USA aut Archives of Toxicology Springer-Verlag 85/6(2011-06-01), 681-687 0340-5761 85:6<681 2011 85 204 https://doi.org/10.1007/s00204-010-0603-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0603-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Williams Gary New York Medical College, 10595, Valhalla, NY, USA aut NATIONALLICENCE 700 1- Brunnemann Klaus New York Medical College, 10595, Valhalla, NY, USA aut NATIONALLICENCE 700 1- Iatropoulos Michael New York Medical College, 10595, Valhalla, NY, USA aut NATIONALLICENCE 700 1- Smart Daniel New York Medical College, 10595, Valhalla, NY, USA aut NATIONALLICENCE 700 1- Enzmann Harald New York Medical College, 10595, Valhalla, NY, USA aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/6(2011-06-01), 681-687 0340-5761 85:6<681 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer