caa a22 4500 44585278X CHVBK 20180317145418.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00204-010-0605-x doi (NATIONALLICENCE)springer-10.1007/s00204-010-0605-x Excretion of 2,3-dihydroxy-propionamide (OH-PA), the hydrolysis product of glycidamide, in human urine after single oral dose of deuterium-labeled acrylamide [Elektronische Daten] [Eva Hartmann, Julia Latzin, Birgit Schindler, Holger Koch, Jürgen Angerer] A dose of 0.99mg d3-acrylamide (d3-AA) (13.2μg/kg body weight) was ingested by a healthy male volunteer. Urine samples were collected over a period of 46h after the intake and analyzed for the hydrolysis product of glycidamide (GA), 2,3-dihydroxy-propionamide (OH-PA), a metabolite of the toxicologically relevant oxidative AA metabolism pathway; 5.4% of the administered d3-AA dose was eliminated as OH-PA within 46h after ingestion. Therefore, OH-PA represents a major metabolite of the oxidative metabolism pathway. Elimination kinetics of OH-PA is similar to the oxidative metabolites N-acetyl-S-(2-carbamoyl-2-hydroxyethyl)-cysteine (GAMA) and N-acetyl-S-(1-carbamoyl-2-hydroxyethyl)-cysteine (iso-GAMA). The major excretion of d3-OH-PA took place between 8 and 22h with the highest urinary d3-OH-PA concentration (c max) of 69.3μg/L urine, 18h (t max) postdose. OH-PA (5.4%), together with the other known urinary metabolites of the oxidative pathway GAMA (4.6%) and iso-GAMA (0.8%), represents 10.8% of the total AA dose. The share of the oxidative pathway metabolites is much smaller than the share of the reductive pathway metabolite N-acetyl-S-(2-carbamoylethyl)-cysteine (AAMA) that represents 51.7% of the ingested d3-AA dose. However, this new quantitative human data on OH-PA together with the previous data on the other oxidative pathway metabolites are of special importance when evaluating the carcinogenic potential of AA and when comparing human data with data from animal studies. Springer-Verlag, 2010 Acrylamide (AA) nationallicence Glycidamide (GA) nationallicence Biological monitoring nationallicence 2,3-Dihydroxy-propionamide (OH-PA) nationallicence Metabolism nationallicence Toxicokinetics nationallicence Hartmann Eva Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, University of Erlangen-Nürnberg, Schillerstr. 25/29, 91054, Erlangen, Germany aut Latzin Julia Institute for Prevention and Occupational Medicine of the German Social Accident Insurance - Institute of the Ruhr-Universität Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut Schindler Birgit Institute for Prevention and Occupational Medicine of the German Social Accident Insurance - Institute of the Ruhr-Universität Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut Koch Holger Institute for Prevention and Occupational Medicine of the German Social Accident Insurance - Institute of the Ruhr-Universität Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut Angerer Jürgen Institute for Prevention and Occupational Medicine of the German Social Accident Insurance - Institute of the Ruhr-Universität Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut Archives of Toxicology Springer-Verlag 85/6(2011-06-01), 601-606 0340-5761 85:6<601 2011 85 204 https://doi.org/10.1007/s00204-010-0605-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0605-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hartmann Eva Institute and Outpatient Clinic of Occupational, Social and Environmental Medicine, University of Erlangen-Nürnberg, Schillerstr. 25/29, 91054, Erlangen, Germany aut NATIONALLICENCE 700 1- Latzin Julia Institute for Prevention and Occupational Medicine of the German Social Accident Insurance - Institute of the Ruhr-Universität Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut NATIONALLICENCE 700 1- Schindler Birgit Institute for Prevention and Occupational Medicine of the German Social Accident Insurance - Institute of the Ruhr-Universität Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut NATIONALLICENCE 700 1- Koch Holger Institute for Prevention and Occupational Medicine of the German Social Accident Insurance - Institute of the Ruhr-Universität Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut NATIONALLICENCE 700 1- Angerer Jürgen Institute for Prevention and Occupational Medicine of the German Social Accident Insurance - Institute of the Ruhr-Universität Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/6(2011-06-01), 601-606 0340-5761 85:6<601 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer