caa a22 4500 445852836 CHVBK 20180317145418.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00204-010-0639-0 doi (NATIONALLICENCE)springer-10.1007/s00204-010-0639-0 Shiga toxin type 2 (Stx2), a potential agent of bioterrorism, has a short distribution and a long elimination half-life, and induces kidney and thymus lesions in rats [Elektronische Daten] [Yue-Nan Liu, Sheng-han Wang, Tao Li, Qin Wang, Wei Tu, Kun Cai, Xiao-Jun Hou, Ren-Mao Tian, Xiang Gao, Hao Liu, Le Xiao, Jing Shi, Yuan-Guo Cheng, Jian-Chun Li, Hui Wang] Shiga toxin type 2, a major virulence factor produced by the Shiga toxin-producing Escherichia coli, is a potential toxin agent of bioterrorism. In this study, iodine-125 (125I) was used as an indicator to describe the in vivo Stx2 biodistribution profile. The rats were injected intravenously (i.v.) with 125I-Stx2 at three doses of 5.1-127.5μg/kg body weight. Stx2 had a short distribution half-life (t 1/2α, less than 6min) and a long elimination half-life in rat. The toxicokinetics of Stx2 in rats was dose dependent and nonlinear. Stx2 concentrations in various tissues were detected at 5-min, 0.5-h, and 72-h postinjection. High radioactivity was found in the lungs, kidneys, nasal turbinates, and sometimes in the eyes, which has never been reported in previous studies. In a preliminary assessment, lesions were found in the kidney and thymus. Springer-Verlag, 2011 Shiga toxin type 2 nationallicence Toxicokinetics nationallicence Tissue distribution nationallicence bioterrorism nationallicence Liu Yue-Nan Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut Wang Sheng-han Department of Urology, Beijing Xuanwu Hospital, Beijing, People's Republic of China aut Li Tao Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut Wang Qin Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut Tu Wei Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut Cai Kun Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut Hou Xiao-Jun Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut Tian Ren-Mao Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut Gao Xiang Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut Liu Hao Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut Xiao Le Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut Shi Jing Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut Cheng Yuan-Guo Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut Li Jian-Chun Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut Wang Hui Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut Archives of Toxicology Springer-Verlag 85/9(2011-09-01), 1133-1140 0340-5761 85:9<1133 2011 85 204 https://doi.org/10.1007/s00204-010-0639-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0639-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Yue-Nan Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wang Sheng-han Department of Urology, Beijing Xuanwu Hospital, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Li Tao Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wang Qin Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Tu Wei Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Cai Kun Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Hou Xiao-Jun Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Tian Ren-Mao Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Gao Xiang Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Liu Hao Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Xiao Le Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Shi Jing Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Cheng Yuan-Guo Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Li Jian-Chun Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wang Hui Department of Microbiological Omics and Bioinformatics, State Key Laboratory of Pathogens and Biosecurity, Beijing Institute of Microbiology and Epidemiology, No.20 Dongdajie, Fengtai District, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/9(2011-09-01), 1133-1140 0340-5761 85:9<1133 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer