caa a22 4500 445852917 CHVBK 20180317145419.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00204-010-0638-1 doi (NATIONALLICENCE)springer-10.1007/s00204-010-0638-1 Life stage-related differences in susceptibility to acrylamide-induced neural and testicular toxicity [Elektronische Daten] [Miwa Takahashi, Kaoru Inoue, Naoki Koyama, Midori Yoshida, Kaoru Irie, Tomomi Morikawa, Makoto Shibutani, Masamitsu Honma, Akiyoshi Nishikawa] In order to assess age-dependence of susceptibility to acrylamide (ACR)-induced neural and testicular toxicity, 3- and 7-week-old male SD rats were given ACR at 0, 50, 100, or 200ppm in the drinking water for 4weeks, and the nervous and male reproductive systems were examined histopathologically. Testicular genotoxicity was evaluated with the comet assay and the micronucleus (MN) test. Glutathione S-transferase (GST) activity and glutathione (GSH) content in the liver and testis were also measured. In both young and adult animals, neurotoxicity was evident from 100ppm and increased in proportion to ACR intake per body weight. In the testis, marked degeneration and exfoliation, mainly of spermatids, were observed from 100ppm limited to young animals. The comet assay revealed ACR to significantly induce DNA damage from 100ppm in both life stages, while MNs were found only in young rats from 100ppm. The level of GST activity in the testis of young rats at the end of experiment was significantly lower than that of adult animals, regardless of the ACR treatment. There were no life stage-related differences in GSH contents in the liver and testis. These results suggest that susceptibility to neurotoxicity might not differ between young and adult rats when exposure levels are adjusted for body weight. Regarding testicular toxicity, young animals around puberty proved more susceptible than adult animals, possibly due to their lower level of testicular GST activity than that in adult animals. Springer-Verlag, 2011 Acrylamide nationallicence Age nationallicence Susceptibility nationallicence Neurotoxicity nationallicence Testicular toxicity nationallicence Rat nationallicence Takahashi Miwa Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut Inoue Kaoru Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut Koyama Naoki Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1-18-1 Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut Yoshida Midori Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut Irie Kaoru Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut Morikawa Tomomi Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut Shibutani Makoto Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut Honma Masamitsu Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1-18-1 Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut Nishikawa Akiyoshi Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut Archives of Toxicology Springer-Verlag 85/9(2011-09-01), 1109-1120 0340-5761 85:9<1109 2011 85 204 https://doi.org/10.1007/s00204-010-0638-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0638-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takahashi Miwa Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Inoue Kaoru Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Koyama Naoki Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1-18-1 Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Yoshida Midori Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Irie Kaoru Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Morikawa Tomomi Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Shibutani Makoto Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Honma Masamitsu Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1-18-1 Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut NATIONALLICENCE 700 1- Nishikawa Akiyoshi Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, 158-8501, Setagaya-ku, Tokyo, Japan aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/9(2011-09-01), 1109-1120 0340-5761 85:9<1109 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer