caa a22 4500 445852925 CHVBK 20180317145419.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00204-011-0655-8 doi (NATIONALLICENCE)springer-10.1007/s00204-011-0655-8 A single intratracheal instillation of single-walled carbon nanotubes induced early lung fibrosis and subchronic tissue damage in mice [Elektronische Daten] [Eun-Jung Park, Jinkyu Roh, Soo-Nam Kim, Min-sung Kang, Young-Ah Han, Younghun Kim, Jin Hong, Kyunghee Choi] Large amounts of nanomaterials may reach both the natural and occupational environments. This represents a potential health hazard. People have forecasted that CNTs may lead to the toxicity such as mesothelioma and fibrosis like asbestos. To identify dominant immune responses induced by SWCNTs, we investigated the composition of bronchioalveolar lavage (BAL) cells, the secretion of cytokine and collagen, histopathology, protein expression, and cell phenotypes over time after a single administration of single-walled carbon nanotubes (SWCNT). In our results, the number of total cells and macrophages remained at the up-regulated level until Day 28, neutrophils rapidly increased at Day 1, and lymphocytes increased from Day 7. In the BAL fluid, pro-inflammatory cytokines rapidly increased at Day 1 and remained at an up-regulated level throughout the experimental period. IL-12 and IL-10 rapidly increased at Day 1 after administration and remained at a similar level until Day 28. IFN-γ and IL-4 reached the maximum at Day 1, and IL-5, TGF-β, and collagen reached the maximum at Day 7. IL-13 and IL-17 increased in a time-dependent manner. The distribution of B cells and cytotoxic T cells markedly increased at Days 7 and 14, and fibrotic lesions were histopathologically observed at Days 7 and 14. The expressions of caspase-3, p53, COL1A1, COX-2, iNOS, MMP-9, and MMP-2 were also markedly increased at Days 7 and 14. In addition, the expression of mesothelin, iNOS, MMP-9, and p53 was up-regulated until Day 28. Based on these findings, we suggest that a single intratracheal instillation of SWCNTs may induce early lung fibrosis and subchronic tissue damage. Springer-Verlag, 2011 SWCNT nationallicence Lung fibrosis nationallicence Mesothelin nationallicence Cytokine nationallicence Inflammation nationallicence Park Eun-Jung Environmental Health Risk Research Department, National Institute of Environmental Research, Kyungseo-dong, Seo-gu, 404-708, Incheon, Korea aut Roh Jinkyu Department of Chemical Engineering, Kwangwoon University, 447-1, Wolgye-dong, Nowon-gu, 139-701, Seoul, Korea aut Kim Soo-Nam Inhalation Toxicology Center, KIT Jeongeup Campus, 1051, Shinjeong-dong, Jeongeup, Korea aut Kang Min-sung Inhalation Toxicology Center, KIT Jeongeup Campus, 1051, Shinjeong-dong, Jeongeup, Korea aut Han Young-Ah Inhalation Toxicology Center, KIT Jeongeup Campus, 1051, Shinjeong-dong, Jeongeup, Korea aut Kim Younghun Department of Chemical Engineering, Kwangwoon University, 447-1, Wolgye-dong, Nowon-gu, 139-701, Seoul, Korea aut Hong Jin College of Pharmacy and Medical Research Center, Chungbuk National University, 410, Seongbong-ro, Heungdeok-gu, 361-763, Cheongju, Chungbuk, Korea aut Choi Kyunghee Environmental Health Risk Research Department, National Institute of Environmental Research, Kyungseo-dong, Seo-gu, 404-708, Incheon, Korea aut Archives of Toxicology Springer-Verlag 85/9(2011-09-01), 1121-1131 0340-5761 85:9<1121 2011 85 204 https://doi.org/10.1007/s00204-011-0655-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-011-0655-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Park Eun-Jung Environmental Health Risk Research Department, National Institute of Environmental Research, Kyungseo-dong, Seo-gu, 404-708, Incheon, Korea aut NATIONALLICENCE 700 1- Roh Jinkyu Department of Chemical Engineering, Kwangwoon University, 447-1, Wolgye-dong, Nowon-gu, 139-701, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Soo-Nam Inhalation Toxicology Center, KIT Jeongeup Campus, 1051, Shinjeong-dong, Jeongeup, Korea aut NATIONALLICENCE 700 1- Kang Min-sung Inhalation Toxicology Center, KIT Jeongeup Campus, 1051, Shinjeong-dong, Jeongeup, Korea aut NATIONALLICENCE 700 1- Han Young-Ah Inhalation Toxicology Center, KIT Jeongeup Campus, 1051, Shinjeong-dong, Jeongeup, Korea aut NATIONALLICENCE 700 1- Kim Younghun Department of Chemical Engineering, Kwangwoon University, 447-1, Wolgye-dong, Nowon-gu, 139-701, Seoul, Korea aut NATIONALLICENCE 700 1- Hong Jin College of Pharmacy and Medical Research Center, Chungbuk National University, 410, Seongbong-ro, Heungdeok-gu, 361-763, Cheongju, Chungbuk, Korea aut NATIONALLICENCE 700 1- Choi Kyunghee Environmental Health Risk Research Department, National Institute of Environmental Research, Kyungseo-dong, Seo-gu, 404-708, Incheon, Korea aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/9(2011-09-01), 1121-1131 0340-5761 85:9<1121 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer