caa a22 4500 445852941 CHVBK 20180317145419.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s00204-011-0646-9 doi (NATIONALLICENCE)springer-10.1007/s00204-011-0646-9 Prevention of benzene-induced genotoxicity in bone marrow and lung cells: superiority of polyphenolic acetates to polyphenols [Elektronische Daten] [Ajit Kumar, Anupam Sushama, Vishwajeet Rohil, Sushma Manral, Sukanya Gangopadhyay, Ashok Prasad, Hanumantharao Raj, Virender Parmar] Previous investigations carried out in our laboratory have highlighted that 7,8-diacetoxy-4-methylcoumarin demonstrates a mechanism-based inhibition of cytochrome P450 (Cyt-P450) activities such as microsome-mediated aflatoxin B1 (AFB1) epoxidation, dealkylation of alkylated resorufin, and toxicokinetics of benzene. 7,8-Diacetoxy-4-methylcoumarin, quercetin pentaacetate, and ellagic acid peracetate were also found to be effective in giving the protection of AFB1-induced genotoxicity in rat's bone marrow and lung cells possibly due to acetylation of Cyt-P450 apoprotein mediated by acetoxy drug: protein transacetylase. Later, this transacetylase was identified as calreticulin, and the acetyltransferase function of calreticulin was appropriately termed calreticulin transacetylase. In this communication, we have focused on the superiority of several classes of polyphenolic acetates to polyphenols in the modification of Cyt-P450-linked mixed function oxidases (MFOs) such as 7-ethoxyresorufin O-deethylase (EROD) and pentoxyresorufin O-dealkylase (PROD). Special attention has also been focused on benzene-induced genotoxicity in bone marrow and lung cells. Results clearly indicated that polyphenolic acetates demonstrated time-dependent inhibition of Cyt-P450-linked MFOs, while parent polyphenols failed to demonstrate the same. Polyphenolic acetates were found to be more superior to polyphenols in preventing benzene-induced micronuclei formation. The pattern of inhibition of Cyt-P450-dependent MFOs and benzene-induced micronuclei formation by polyphenolic acetates was found in tune with their specificities to calreticulin transacetylase. These results further substantiated that inhibition of Cyt-P450-linked MFOs and benzene-induced genotoxicity in bone marrow and lung cells by polyphenolic acetates are mediated by the action of calreticulin transacetylase that catalyzes the acetylation of concerned proteins. Springer-Verlag, 2011 Benzene nationallicence Micronuclei nationallicence Genotoxicity nationallicence Polyphenolic acetates nationallicence Calreticulin transacetylase nationallicence Kumar Ajit Department of Biochemistry, V. P. Chest Institute, University of Delhi, 110007, Delhi, India aut Sushama Anupam Department of Biochemistry, V. P. Chest Institute, University of Delhi, 110007, Delhi, India aut Rohil Vishwajeet Department of Biochemistry, V. P. Chest Institute, University of Delhi, 110007, Delhi, India aut Manral Sushma Department of Biochemistry, V. P. Chest Institute, University of Delhi, 110007, Delhi, India aut Gangopadhyay Sukanya Department of Biochemistry, V. P. Chest Institute, University of Delhi, 110007, Delhi, India aut Prasad Ashok Department of Chemistry, Bioorganic Laboratory, University of Delhi, 110007, Delhi, India aut Raj Hanumantharao Department of Biochemistry, V. P. Chest Institute, University of Delhi, 110007, Delhi, India aut Parmar Virender Department of Chemistry, Bioorganic Laboratory, University of Delhi, 110007, Delhi, India aut Archives of Toxicology Springer-Verlag 85/9(2011-09-01), 1141-1150 0340-5761 85:9<1141 2011 85 204 https://doi.org/10.1007/s00204-011-0646-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-011-0646-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kumar Ajit Department of Biochemistry, V. P. Chest Institute, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Sushama Anupam Department of Biochemistry, V. P. Chest Institute, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Rohil Vishwajeet Department of Biochemistry, V. P. Chest Institute, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Manral Sushma Department of Biochemistry, V. P. Chest Institute, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Gangopadhyay Sukanya Department of Biochemistry, V. P. Chest Institute, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Prasad Ashok Department of Chemistry, Bioorganic Laboratory, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Raj Hanumantharao Department of Biochemistry, V. P. Chest Institute, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 700 1- Parmar Virender Department of Chemistry, Bioorganic Laboratory, University of Delhi, 110007, Delhi, India aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/9(2011-09-01), 1141-1150 0340-5761 85:9<1141 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer