caa a22 4500 445853026 CHVBK 20180317145419.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00204-011-0644-y doi (NATIONALLICENCE)springer-10.1007/s00204-011-0644-y Nanosized TiO2 caused minor airflow limitation in the murine airways [Elektronische Daten] [Maija Leppänen, Anne Korpi, Mirella Miettinen, Jani Leskinen, Tiina Torvela, Elina Rossi, Esa Vanhala, Henrik Wolff, Harri Alenius, Veli-Matti Kosma, Jorma Joutsensaari, Jorma Jokiniemi, Pertti Pasanen] The use of nanotechnology is increasing exponentially, whereas the possible adverse health effects of engineered nanoparticles (NPs) are so far less known. Standardized mouse bioassay was used to study sensory and pulmonary irritation, airflow limitation, and inflammation potency of nanosized TiO2. Single exposure (0.5h) to in situ generated TiO2 (primary particle size 20nm; geometric mean diameters of 91, 113, and 130nm at mass concentrations of 8, 20, and 30mg/m3, respectively; crystal phase anatase+brookite (3:1)) caused airflow limitation in the conducting airways at each studied exposure concentration, which was shown as a reduction in expiratory flow, being at the lowest 73% of baseline. The response was not dose dependent. Repeated exposures (altogether 16h, 1h/day, 4days/week for 4weeks) to TiO2 at mass concentration of 30mg/m3 caused as intense airflow limitation effect as the single exposures, and the extent of the responses stayed about the same along the exposure days. Sensory irritation was fairly minor. Pulmonary irritation was more pronounced during the latter part of the repeated exposures compared to the single exposures and the beginning of the repeated exposures. Sensory and pulmonary irritation were observed also in the control group, and, therefore, reaction by-products (NO2 and C3H6) may have contributed to the irritation effects. TiO2 NPs accumulated mainly in the pulmonary macrophages, and they did not cause nasal or pulmonary inflammation. In conclusion, the irritation and inflammation potencies of studied TiO2 seemed to be low. Springer-Verlag, 2011 Titanium dioxide nationallicence Engineered nanoparticles nationallicence Airflow limitation nationallicence Irritation nationallicence Inflammation nationallicence Leppänen Maija Department of Environmental Science, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland aut Korpi Anne Department of Environmental Science, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland aut Miettinen Mirella Department of Environmental Science, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland aut Leskinen Jani Department of Environmental Science, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland aut Torvela Tiina Department of Environmental Science, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland aut Rossi Elina Finnish Institute of Occupational Health, Unit of Excellence in Immunotoxicology, Topeliuksenkatu 41 a A, 00250, Helsinki, Finland aut Vanhala Esa Finnish Institute of Occupational Health, Aerosols, Dusts and Metals, Topeliuksenkatu 41 a A, 00250, Helsinki, Finland aut Wolff Henrik Finnish Institute of Occupational Health, Biological Mechanisms and Prevention of Work-Related Diseases/Immunopathology, Topeliuksenkatu 41 a A, 00250, Helsinki, Finland aut Alenius Harri Finnish Institute of Occupational Health, Unit of Excellence in Immunotoxicology, Topeliuksenkatu 41 a A, 00250, Helsinki, Finland aut Kosma Veli-Matti Department of Pathology and Forensic Medicine, Faculty of Health Sciences, School of Medicine, Institute of Clinical Medicine, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland aut Joutsensaari Jorma Department of Physics and Mathematics, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland aut Jokiniemi Jorma Department of Environmental Science, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland aut Pasanen Pertti Department of Environmental Science, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland aut Archives of Toxicology Springer-Verlag 85/7(2011-07-01), 827-839 0340-5761 85:7<827 2011 85 204 https://doi.org/10.1007/s00204-011-0644-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-011-0644-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Leppänen Maija Department of Environmental Science, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland aut NATIONALLICENCE 700 1- Korpi Anne Department of Environmental Science, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland aut NATIONALLICENCE 700 1- Miettinen Mirella Department of Environmental Science, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland aut NATIONALLICENCE 700 1- Leskinen Jani Department of Environmental Science, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland aut NATIONALLICENCE 700 1- Torvela Tiina Department of Environmental Science, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland aut NATIONALLICENCE 700 1- Rossi Elina Finnish Institute of Occupational Health, Unit of Excellence in Immunotoxicology, Topeliuksenkatu 41 a A, 00250, Helsinki, Finland aut NATIONALLICENCE 700 1- Vanhala Esa Finnish Institute of Occupational Health, Aerosols, Dusts and Metals, Topeliuksenkatu 41 a A, 00250, Helsinki, Finland aut NATIONALLICENCE 700 1- Wolff Henrik Finnish Institute of Occupational Health, Biological Mechanisms and Prevention of Work-Related Diseases/Immunopathology, Topeliuksenkatu 41 a A, 00250, Helsinki, Finland aut NATIONALLICENCE 700 1- Alenius Harri Finnish Institute of Occupational Health, Unit of Excellence in Immunotoxicology, Topeliuksenkatu 41 a A, 00250, Helsinki, Finland aut NATIONALLICENCE 700 1- Kosma Veli-Matti Department of Pathology and Forensic Medicine, Faculty of Health Sciences, School of Medicine, Institute of Clinical Medicine, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland aut NATIONALLICENCE 700 1- Joutsensaari Jorma Department of Physics and Mathematics, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland aut NATIONALLICENCE 700 1- Jokiniemi Jorma Department of Environmental Science, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland aut NATIONALLICENCE 700 1- Pasanen Pertti Department of Environmental Science, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/7(2011-07-01), 827-839 0340-5761 85:7<827 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer