caa a22 4500 445853069 CHVBK 20180317145419.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00204-010-0574-0 doi (NATIONALLICENCE)springer-10.1007/s00204-010-0574-0 Aspect ratio has no effect on genotoxicity of multi-wall carbon nanotubes [Elektronische Daten] [Jin Kim, Kyu Lee, Young Lee, Hyun Cho, Ki Kim, Kyung Choi, Sang Lee, Kyung Song, Chang Kang, Il Yu] Carbon nanotubes (CNTs) have specific physico-chemical and electrical properties that are useful for telecommunications, medicine, materials, manufacturing processes and the environmental and energy sectors. Yet, despite their many advantages, it is also important to determine whether CNTs may represent a hazard to the environment and human health. Like asbestos, the aspect ratio (length:diameter) and metal components of CNTs are known to have an effect on the toxicity of carbon nanotubes. Thus, to evaluate the toxic potential of CNTs in relation to their aspect ratio and metal contamination, in vivo and in vitro genotoxicity tests were conducted using high-aspect-ratio (diameter: 10-15nm, length: ~10μm) and low-aspect-ratio multi-wall carbon nanotubes (MWCNTs, diameter: 10-15nm, length: ~150nm) according to OECD test guidelines 471 (bacterial reverse mutation test), 473 (in vitro chromosome aberration test), and 474 (in vivo micronuclei test) with a good laboratory practice system. To determine the treatment concentration for all the tests, a solubility and dispersive test was performed, and a 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) solution found to be more suitable than distilled water. Neither the high- nor the low-aspect-ratio MWCNTs induced any genotoxicity in a bacterial reverse mutation test (~1,000μg/plate), in vitro chromosome aberration test (without S9: ~6.25μg/ml, with S9: ~50μg/ml), or in vivo micronuclei test (~50mg/kg). However, the high-aspect-ratio MWCNTs were found to be more toxic than the low-aspect-ratio MWCNTs. Thus, while high-aspect-ratio MWCNTs do not induce direct genotoxicity or metabolic activation-mediated genotoxicity, genotoxicity could still be induced indirectly through oxidative stress or inflammation. Springer-Verlag, 2010 Carbon nanotubes (CNTs) nationallicence Multi-wall carbon nanotubes (MWCNTs) nationallicence Genotoxicity nationallicence OECD test guidelines nationallicence Bacterial reverse mutation test nationallicence In vitro chromosome aberration test nationallicence In vivo micronuclei test nationallicence Good laboratory practice (GLP) nationallicence Cytotoxicity nationallicence Kim Jin Biosafety Evaluation Headquarter, Korea Environment and Merchandise Testing Institute, Incheon, South Korea aut Lee Kyu Department of Physics, Center for Nanotubes and Nanostructured Composites, Sungkyunkwan Advanced Institute of Nanotechnology, Sungkyunkwan University, Suwon, South Korea aut Lee Young Department of Physics, Center for Nanotubes and Nanostructured Composites, Sungkyunkwan Advanced Institute of Nanotechnology, Sungkyunkwan University, Suwon, South Korea aut Cho Hyun Biosafety Evaluation Headquarter, Korea Environment and Merchandise Testing Institute, Incheon, South Korea aut Kim Ki Biosafety Evaluation Headquarter, Korea Environment and Merchandise Testing Institute, Incheon, South Korea aut Choi Kyung Risk Assessment Division, National Institute of Environmental Research, Incheon, South Korea aut Lee Sang Risk Assessment Division, National Institute of Environmental Research, Incheon, South Korea aut Song Kyung Biosafety Evaluation Headquarter, Korea Environment and Merchandise Testing Institute, Incheon, South Korea aut Kang Chang Fusion Technology Research Institute, Hoseo University, 165 Sechul-ri, Baebang-myun, 336-795, Asan, South Korea aut Yu Il Biosafety Evaluation Headquarter, Korea Environment and Merchandise Testing Institute, Incheon, South Korea aut Archives of Toxicology Springer-Verlag 85/7(2011-07-01), 775-786 0340-5761 85:7<775 2011 85 204 https://doi.org/10.1007/s00204-010-0574-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0574-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kim Jin Biosafety Evaluation Headquarter, Korea Environment and Merchandise Testing Institute, Incheon, South Korea aut NATIONALLICENCE 700 1- Lee Kyu Department of Physics, Center for Nanotubes and Nanostructured Composites, Sungkyunkwan Advanced Institute of Nanotechnology, Sungkyunkwan University, Suwon, South Korea aut NATIONALLICENCE 700 1- Lee Young Department of Physics, Center for Nanotubes and Nanostructured Composites, Sungkyunkwan Advanced Institute of Nanotechnology, Sungkyunkwan University, Suwon, South Korea aut NATIONALLICENCE 700 1- Cho Hyun Biosafety Evaluation Headquarter, Korea Environment and Merchandise Testing Institute, Incheon, South Korea aut NATIONALLICENCE 700 1- Kim Ki Biosafety Evaluation Headquarter, Korea Environment and Merchandise Testing Institute, Incheon, South Korea aut NATIONALLICENCE 700 1- Choi Kyung Risk Assessment Division, National Institute of Environmental Research, Incheon, South Korea aut NATIONALLICENCE 700 1- Lee Sang Risk Assessment Division, National Institute of Environmental Research, Incheon, South Korea aut NATIONALLICENCE 700 1- Song Kyung Biosafety Evaluation Headquarter, Korea Environment and Merchandise Testing Institute, Incheon, South Korea aut NATIONALLICENCE 700 1- Kang Chang Fusion Technology Research Institute, Hoseo University, 165 Sechul-ri, Baebang-myun, 336-795, Asan, South Korea aut NATIONALLICENCE 700 1- Yu Il Biosafety Evaluation Headquarter, Korea Environment and Merchandise Testing Institute, Incheon, South Korea aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/7(2011-07-01), 775-786 0340-5761 85:7<775 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer