caa a22 4500 445853107 CHVBK 20180317145419.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s00204-010-0627-4 doi (NATIONALLICENCE)springer-10.1007/s00204-010-0627-4 Differential stability of lead sulfide nanoparticles influences biological responses in embryonic zebrafish [Elektronische Daten] [Lisa Truong, Ian Moody, Dylan Stankus, Jeffrey Nason, Mark Lonergan, Robert Tanguay] As the number of nanoparticle-based products increase in the marketplace, there will be increased potential for human exposures to these engineered materials throughout the product life cycle. We currently lack sufficient data to understand or predict the inherent nanomaterial characteristics that drive nanomaterial-biological interactions and responses. In this study, we utilized the embryonic zebrafish (Danio rerio) model to investigate the importance of nanoparticle (NP) surface functionalization, in particular as it pertains to nanoparticle stability, on in vivo biological responses. This is a comparative study where two lead sulfide nanoparticles (PbS-NPs) with nearly identical core sizes, but functionalized with either sodium 3-mercaptopropanesulfonate (MT) or sodium 2,3-dimercaptopropanesulfonate (DT) ligand, were used. Developmental exposures and assessments revealed differential biological responses to these engineered nanoparticles. Exposures beginning at 6h post fertilization (hpf) to MT-functionalized nanoparticles (PbS-MT) led to 100% mortality by 120 hpf while exposure to DT-functionalized nanoparticles (PbS-DT) produced less than a 5% incident in mortality at the same concentration. Exposure to the MT and DT ligands themselves did not produce adverse developmental effects when not coupled to the NP core. Following exposure, we confirmed that the embryos took up both PbS-MT and PbS-DT material using inductively coupled plasma-mass spectrometry (ICP-MS). The stability of the nanoparticles in the aqueous solution was also characterized. The nanoparticles decompose and precipitate upon exposure to air. Soluble lead ions were observed following nanoparticle precipitation and in greater concentration for the PbS-MT sample compared to the PbS-DT sample. These studies demonstrate that in vivo assessments can be effectively used to characterize the role of NP surface functionalization in predicting biological responses. Springer-Verlag, 2010 Lead sulfide nationallicence Nanoparticle nationallicence Nanomaterial-biological interaction nationallicence Toxicity nationallicence Stability nationallicence Truong Lisa Nanotoxicology Laboratory, Department of Environmental and Molecular Toxicology, ALS 1007, Oregon State University, 97331, Corvallis, OR, USA aut Moody Ian Department of Chemistry and the Materials Science Institute, University of Oregon, 97403, Eugene, OR, USA aut Stankus Dylan Safer Nanomaterials and Nanomanufacturing Initiative, Oregon Nanoscience and Microtechnologies Institute, Corvallis, OR, USA aut Nason Jeffrey Safer Nanomaterials and Nanomanufacturing Initiative, Oregon Nanoscience and Microtechnologies Institute, Corvallis, OR, USA aut Lonergan Mark Safer Nanomaterials and Nanomanufacturing Initiative, Oregon Nanoscience and Microtechnologies Institute, Corvallis, OR, USA aut Tanguay Robert Nanotoxicology Laboratory, Department of Environmental and Molecular Toxicology, ALS 1007, Oregon State University, 97331, Corvallis, OR, USA aut Archives of Toxicology Springer-Verlag 85/7(2011-07-01), 787-798 0340-5761 85:7<787 2011 85 204 https://doi.org/10.1007/s00204-010-0627-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0627-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Truong Lisa Nanotoxicology Laboratory, Department of Environmental and Molecular Toxicology, ALS 1007, Oregon State University, 97331, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Moody Ian Department of Chemistry and the Materials Science Institute, University of Oregon, 97403, Eugene, OR, USA aut NATIONALLICENCE 700 1- Stankus Dylan Safer Nanomaterials and Nanomanufacturing Initiative, Oregon Nanoscience and Microtechnologies Institute, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Nason Jeffrey Safer Nanomaterials and Nanomanufacturing Initiative, Oregon Nanoscience and Microtechnologies Institute, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Lonergan Mark Safer Nanomaterials and Nanomanufacturing Initiative, Oregon Nanoscience and Microtechnologies Institute, Corvallis, OR, USA aut NATIONALLICENCE 700 1- Tanguay Robert Nanotoxicology Laboratory, Department of Environmental and Molecular Toxicology, ALS 1007, Oregon State University, 97331, Corvallis, OR, USA aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/7(2011-07-01), 787-798 0340-5761 85:7<787 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer