caa a22 4500 44585314X CHVBK 20180317145419.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00204-011-0722-1 doi (NATIONALLICENCE)springer-10.1007/s00204-011-0722-1 Evaluation of the cytotoxic and inflammatory potential of differentially shaped zinc oxide nanoparticles [Elektronische Daten] [Boon Heng, Xinxin Zhao, Eng Tan, Nurulain Khamis, Aarti Assodani, Sijing Xiong, Christiane Ruedl, Kee Ng, Joachim Loo] Zinc oxide (ZnO) nanoparticles have wide-ranging applications in a diverse array of industrial and consumer products, from ceramic manufacture and paint formulation to sunscreens and haircare products. Hence, it is imperative to rigorously characterize the health and safety aspects of human exposure to ZnO nanoparticles. This study therefore evaluated the cellular association, cytotoxic and inflammatory potential of spherical and sheet-shaped ZnO nanoparticles (of approximately the same specific surface area ≈30cm2/g) on mouse and human cell lines (RAW-264.7 and BEAS-2B respectively), as well as with primary cultures of mouse bone marrow-derived dendritic cells (DC). The WST-8 assay demonstrated dose-dependent effects on the cytotoxicity of spherical and sheet-shaped ZnO nanoparticles on both RAW-264.7 and BEAS-2B cells, even though there was no significant effect of shape on the cytotoxicity of ZnO nanoparticles. There was however higher cellular association of spherical versus sheet-shaped ZnO nanoparticles. Measurement of reactive oxygen species (ROS) with the 2′,7′-dichlorfluorescein-diacetate (DCFH-DA) assay indicated up to 4-folds increase in ROS level upon exposure to ZnO nanoparticles, but there was again no significant difference between both ZnO nanoparticle shapes. Exposure of primary dendritic cells to ZnO nanoparticles upregulated expression of CD80 and CD86 (well-known markers of DC activation and maturation) and stimulated release of pro-inflammatory cytokines—IL-6 and TNF-α, thus pointing to the potential of ZnO nanoparticles in inducing inflammation. Hence, our study indicated that ZnO nanoparticles can have potential detrimental effects on cells even at dosages where there are little or no observable cytotoxic effects. Springer-Verlag, 2011 Cytotoxicity nationallicence Inflammation nationallicence Nanoparticle nationallicence Oxide nationallicence Zinc nationallicence Heng Boon School of Materials Science and Engineering, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore, Singapore aut Zhao Xinxin School of Materials Science and Engineering, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore, Singapore aut Tan Eng School of Biological Sciences, Nanyang Technological University, Block N4.1, 50 Nanyang Avenue, 639798, Singapore, Singapore aut Khamis Nurulain School of Biological Sciences, Nanyang Technological University, Block N4.1, 50 Nanyang Avenue, 639798, Singapore, Singapore aut Assodani Aarti School of Biological Sciences, Nanyang Technological University, Block N4.1, 50 Nanyang Avenue, 639798, Singapore, Singapore aut Xiong Sijing School of Materials Science and Engineering, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore, Singapore aut Ruedl Christiane School of Biological Sciences, Nanyang Technological University, Block N4.1, 50 Nanyang Avenue, 639798, Singapore, Singapore aut Ng Kee School of Materials Science and Engineering, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore, Singapore aut Loo Joachim School of Materials Science and Engineering, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore, Singapore aut Archives of Toxicology Springer-Verlag 85/12(2011-12-01), 1517-1528 0340-5761 85:12<1517 2011 85 204 https://doi.org/10.1007/s00204-011-0722-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-011-0722-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Heng Boon School of Materials Science and Engineering, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore, Singapore aut NATIONALLICENCE 700 1- Zhao Xinxin School of Materials Science and Engineering, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore, Singapore aut NATIONALLICENCE 700 1- Tan Eng School of Biological Sciences, Nanyang Technological University, Block N4.1, 50 Nanyang Avenue, 639798, Singapore, Singapore aut NATIONALLICENCE 700 1- Khamis Nurulain School of Biological Sciences, Nanyang Technological University, Block N4.1, 50 Nanyang Avenue, 639798, Singapore, Singapore aut NATIONALLICENCE 700 1- Assodani Aarti School of Biological Sciences, Nanyang Technological University, Block N4.1, 50 Nanyang Avenue, 639798, Singapore, Singapore aut NATIONALLICENCE 700 1- Xiong Sijing School of Materials Science and Engineering, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore, Singapore aut NATIONALLICENCE 700 1- Ruedl Christiane School of Biological Sciences, Nanyang Technological University, Block N4.1, 50 Nanyang Avenue, 639798, Singapore, Singapore aut NATIONALLICENCE 700 1- Ng Kee School of Materials Science and Engineering, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore, Singapore aut NATIONALLICENCE 700 1- Loo Joachim School of Materials Science and Engineering, Nanyang Technological University, 60 Nanyang Drive, 637551, Singapore, Singapore aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/12(2011-12-01), 1517-1528 0340-5761 85:12<1517 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer