caa a22 4500 445853220 CHVBK 20180317145420.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00204-011-0698-x doi (NATIONALLICENCE)springer-10.1007/s00204-011-0698-x A new Phaseolus vulgaris lectin induces selective toxicity on human liver carcinoma Hep G2 cells [Elektronische Daten] [Evandro Fang, Wen Pan, Jack Wong, Yau Chan, Xiu Ye, Tzi Ng] We describe here the purification and characterization of a new Phaseolus vulgaris lectin that exhibits selective toxicity to human hepatoma Hep G2 cells and lacks significant toxicity on normal liver WRL 68 cells. This polygalacturonic acid-specific lectin (termed BTKL) was purified from seeds of P. vulgaris cv. Blue tiger king by liquid chromatography techniques. The 60-kDa dimeric lectin showed strong and broad-spectrum hemagglutinating activity toward human, rabbit, rat, and mouse erythrocytes. Bioinformatic analysis unveils substantial N-terminal sequence similarity of BTKL to other Phaseolus lectins. Among a number of tumor cells tested, BTKL exhibits potent anti-Hep G2 activity which is associated with (1) induction of DNA fragmentation, (2) production of apoptotic bodies and chromatin condensation, (3) triggering of cell apoptosis and necrosis, and (4) depolarization of mitochondrial membrane (low ΔΨm). Furthermore, BTKL could induce inducible nitric oxide synthase (iNOS) expression and subsequent nitric oxide production in vitro in mouse macrophages, which may contribute to its antitumor activity. In addition, BTKL could bring about a significant dose-dependent increase in the production of mRNAs of proinflammatory cytokines including interleukin-1 beta, interleukin-2, tumor necrosis factor alpha, and interferon-gamma. In sum, the antitumor activity and mechanism of BTKL provided here suggest that it has potential therapeutic value for human liver cancer. Springer-Verlag, 2011 Liver cancer nationallicence Apoptosis nationallicence Lectin nationallicence Nitric oxide nationallicence Cytokine nationallicence BTKL : Phaseolus vulgaris cv. Blue tiger king lectin nationallicence RT-PCR : Reverse transcription polymerase chain reaction nationallicence SDS-PAGE : Sodium dodecyl sulfate-polyacrylamide gel electrophoresis nationallicence NO : Nitric oxide nationallicence Δ Ψ m : Mitochondrial transmembrane potential nationallicence EDTA : Ethylenediaminetetraacetic acid nationallicence Fang Evandro School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong aut Pan Wen School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong aut Wong Jack School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong aut Chan Yau School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong aut Ye Xiu School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong aut Ng Tzi School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong aut Archives of Toxicology Springer-Verlag 85/12(2011-12-01), 1551-1563 0340-5761 85:12<1551 2011 85 204 https://doi.org/10.1007/s00204-011-0698-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-011-0698-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fang Evandro School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong aut NATIONALLICENCE 700 1- Pan Wen School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong aut NATIONALLICENCE 700 1- Wong Jack School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong aut NATIONALLICENCE 700 1- Chan Yau School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong aut NATIONALLICENCE 700 1- Ye Xiu School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong aut NATIONALLICENCE 700 1- Ng Tzi School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/12(2011-12-01), 1551-1563 0340-5761 85:12<1551 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer