caa a22 4500 445853247 CHVBK 20180317145420.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s00204-011-0717-y doi (NATIONALLICENCE)springer-10.1007/s00204-011-0717-y Role of endogenous hydrogen sulfide on renal damage induced by adriamycin injection [Elektronische Daten] [Heloísa Francescato, Evelyn Marin, Fernando de Queiroz Cunha, Roberto Costa, Cleonice da Silva, Terezila Coimbra] A single injection of adriamycin (ADR) induces marked and persistent proteinuria in rats that progress to glomerular and tubulointerstitial lesions. It has been shown that ADR-induced nephrotoxicity is mediated, at least in part, by oxidative stress that lead to inflammation. Endogenous hydrogen sulfide (H2S) is synthesized from l-cysteine and is an important signaling molecule in inflammation. This study evaluates the effect of DL-propargylglycine (PAG), an inhibitor of endogenous H2S formation, on the evolution of renal damage induced by ADR. The rats were injected i.p. with 0.15M NaCl or PAG (50mg/kg) 2h after ADR injection (3.5mg/kg). Control rats were injected with 0.15M NaCl or PAG only. Twenty hours urine samples were collected for albuminuria and creatinine measurements on days 1 and 14 after saline or ADR injections and on days 2 and 15 blood samples were collected to measure plasma creatinine, then the rats were killed. The kidneys were removed for H2S formation evaluation, renal lipid peroxidation and glutathione levels, and histological and immunohistochemical analysis. On day 2 after ADR injection the rats presented increase in oxidative stress associated with neutrophils and macrophages influx in renal tissue. On day 15 the rats also presented increased desmin expression at glomerular edge and vimentin in cortical tubulointerstitium, as well as albuminuria. All these alterations were reduced by PAG injection. The protective effect of PAG on ADR nephrotoxicity was associated to decreased H2S formation and to restriction of oxidative stress and inflammation in the renal cortex. Springer-Verlag, 2011 Adriamycin nationallicence DL-propargylglycine nationallicence Inflammation nationallicence Renal damage nationallicence Francescato Heloísa Department of Physiology, Faculty of Medicine, University of São Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, São Paulo, Brazil aut Marin Evelyn Department of Physiology, Faculty of Medicine, University of São Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, São Paulo, Brazil aut de Queiroz Cunha Fernando Department of Pharmacology, Faculty of Medicine, University of São Paulo, Ribeirão Preto, SP, Brazil aut Costa Roberto Department of Pathology, Faculty of Medicine, University of São Paulo, Ribeirão Preto, SP, Brazil aut da Silva Cleonice Department of Physiology, Faculty of Medicine, University of São Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, São Paulo, Brazil aut Coimbra Terezila Department of Physiology, Faculty of Medicine, University of São Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, São Paulo, Brazil aut Archives of Toxicology Springer-Verlag 85/12(2011-12-01), 1597-1606 0340-5761 85:12<1597 2011 85 204 https://doi.org/10.1007/s00204-011-0717-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-011-0717-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Francescato Heloísa Department of Physiology, Faculty of Medicine, University of São Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, São Paulo, Brazil aut NATIONALLICENCE 700 1- Marin Evelyn Department of Physiology, Faculty of Medicine, University of São Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, São Paulo, Brazil aut NATIONALLICENCE 700 1- de Queiroz Cunha Fernando Department of Pharmacology, Faculty of Medicine, University of São Paulo, Ribeirão Preto, SP, Brazil aut NATIONALLICENCE 700 1- Costa Roberto Department of Pathology, Faculty of Medicine, University of São Paulo, Ribeirão Preto, SP, Brazil aut NATIONALLICENCE 700 1- da Silva Cleonice Department of Physiology, Faculty of Medicine, University of São Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, São Paulo, Brazil aut NATIONALLICENCE 700 1- Coimbra Terezila Department of Physiology, Faculty of Medicine, University of São Paulo, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, São Paulo, Brazil aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/12(2011-12-01), 1597-1606 0340-5761 85:12<1597 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer