caa a22 4500 44585331X CHVBK 20180317145420.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00204-010-0573-1 doi (NATIONALLICENCE)springer-10.1007/s00204-010-0573-1 Effects of exogenous glutathione on arsenic burden and NO metabolism in brain of mice exposed to arsenite through drinking water [Elektronische Daten] [Yan Wang, Fenghong Zhao, Yaping Jin, Yuan Zhong, Xiaoyun Yu, Gexin Li, Xiuqiang Lv, Guifan Sun] Chronic exposure to inorganic arsenic (iAs) is associated with neurotoxicity. Studies to date have disclosed that methylation of ingested iAs is the main metabolic pathway, and it is a process relying on reduced glutathione (GSH). The aim of this study was to explore the effects of exogenous GSH on arsenic burden and metabolism of nitric oxide (NO) in the brain of mice exposed to arsenite via drinking water. Mice were exposed to sodium arsenite through drinking water contaminated with 50mg/L arsenic for 4weeks and treated intraperitoneally with saline solution, 200mg/kg body weight (b.w), 400mg/kg b.w, or 800mg/kg b.w GSH, respectively, at the 4th week. Levels of iAs, monomethylarsenic acid, and dimethylarsenic acid (DMAs) in the liver, blood, and brain were determined by method of hydride generation coupled with atomic absorption spectrophotometry. Activities of nitric oxide synthase (NOS) and contents of NO in the brain were determined by colorimetric method. Compared with mice exposed to arsenite alone, administration of GSH increased dose-dependently the primary and secondary methylation ratio in the liver, which caused the decrease in percent iAs and increase in percent DMAs in the liver, as a consequence, resulted in significant decrease in iAs levels in the blood and total arsenic levels in both blood and brain. NOS activities and NO levels in the brain of mice in iAs group were significantly lower than those in control; however, administration of GSH could increase significantly activities of NOS and contents of NO. Findings from this study suggested that exogenous GSH could promote both primary and secondary arsenic methylation capacity in the liver, which might facilitate excretion of arsenicals, and consequently reduce arsenic burden in both blood and brain and furthermore ameliorate the effects of arsenicals on NO metabolism in the brain. Springer-Verlag, 2010 Arsenite nationallicence Glutathione nationallicence Arsenic methylation nationallicence Nitric oxide synthase nationallicence Nitric oxide nationallicence Wang Yan Department of Environmental and Occupational Health, School of Public Health, China Medical University, No. 92 North 2nd Road, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut Zhao Fenghong Department of Environmental and Occupational Health, School of Public Health, China Medical University, No. 92 North 2nd Road, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut Jin Yaping Department of Environmental and Occupational Health, School of Public Health, China Medical University, No. 92 North 2nd Road, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut Zhong Yuan Department of Environmental and Occupational Health, School of Public Health, China Medical University, No. 92 North 2nd Road, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut Yu Xiaoyun Department of Environmental and Occupational Health, School of Public Health, China Medical University, No. 92 North 2nd Road, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut Li Gexin Department of Environmental and Occupational Health, School of Public Health, China Medical University, No. 92 North 2nd Road, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut Lv Xiuqiang Department of Environmental and Occupational Health, School of Public Health, China Medical University, No. 92 North 2nd Road, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut Sun Guifan Department of Environmental and Occupational Health, School of Public Health, China Medical University, No. 92 North 2nd Road, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut Archives of Toxicology Springer-Verlag 85/3(2011-03-01), 177-184 0340-5761 85:3<177 2011 85 204 https://doi.org/10.1007/s00204-010-0573-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0573-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Yan Department of Environmental and Occupational Health, School of Public Health, China Medical University, No. 92 North 2nd Road, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Fenghong Department of Environmental and Occupational Health, School of Public Health, China Medical University, No. 92 North 2nd Road, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Jin Yaping Department of Environmental and Occupational Health, School of Public Health, China Medical University, No. 92 North 2nd Road, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Zhong Yuan Department of Environmental and Occupational Health, School of Public Health, China Medical University, No. 92 North 2nd Road, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Yu Xiaoyun Department of Environmental and Occupational Health, School of Public Health, China Medical University, No. 92 North 2nd Road, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Li Gexin Department of Environmental and Occupational Health, School of Public Health, China Medical University, No. 92 North 2nd Road, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Lv Xiuqiang Department of Environmental and Occupational Health, School of Public Health, China Medical University, No. 92 North 2nd Road, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Sun Guifan Department of Environmental and Occupational Health, School of Public Health, China Medical University, No. 92 North 2nd Road, 110001, Heping District, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/3(2011-03-01), 177-184 0340-5761 85:3<177 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer