caa a22 4500 445853352 CHVBK 20180317145420.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00204-010-0578-9 doi (NATIONALLICENCE)springer-10.1007/s00204-010-0578-9 Effect of different buffers on kinetic properties of human acetylcholinesterase and the interaction with organophosphates and oximes [Elektronische Daten] [T. Wille, H. Thiermann, F. Worek] Acetylcholinesterase (AChE) is the primary target of organophosphorus compounds (OP). The investigation into interactions between AChE, OP and oximes in vitro may be affected by the experimental conditions, e.g. by the buffer system. Hence, it was tempting to investigate the Michaelis-Menten kinetics and the inhibition and reactivation kinetics of paraoxon-ethyl, sarin, soman and VX in the presence of phosphate, MOPS, Tyrode and TRIS buffer with human AChE. Compared to phosphate buffer, the inhibition and reactivation kinetics of human erythrocyte AChE were markedly changed by TRIS and in part by MOPS, whereas Tyrode showed similar results to phosphate buffer. These results indicate an effect of the tested buffers on the properties of AChE, and an interaction between OP and oximes has to be considered for the design of in vitro studies and may impair the comparison of data from different laboratories. In view of the comparability of human in vitro kinetic data determined with phosphate buffer with data from human OP poisoning, it seems to be a suitable buffer for the investigation into interactions between AChE, OP and oximes. Springer-Verlag, 2010 Acetylcholinesterase nationallicence Buffer nationallicence Kinetics nationallicence Organophosphorus compound nationallicence Inhibition nationallicence Reactivation nationallicence Wille T. Bundeswehr Institute of Pharmacology and Toxicology, Neuherbergstrasse 11, 80937, Munich, Germany aut Thiermann H. Bundeswehr Institute of Pharmacology and Toxicology, Neuherbergstrasse 11, 80937, Munich, Germany aut Worek F. Bundeswehr Institute of Pharmacology and Toxicology, Neuherbergstrasse 11, 80937, Munich, Germany aut Archives of Toxicology Springer-Verlag 85/3(2011-03-01), 193-198 0340-5761 85:3<193 2011 85 204 https://doi.org/10.1007/s00204-010-0578-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0578-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wille T. Bundeswehr Institute of Pharmacology and Toxicology, Neuherbergstrasse 11, 80937, Munich, Germany aut NATIONALLICENCE 700 1- Thiermann H. Bundeswehr Institute of Pharmacology and Toxicology, Neuherbergstrasse 11, 80937, Munich, Germany aut NATIONALLICENCE 700 1- Worek F. Bundeswehr Institute of Pharmacology and Toxicology, Neuherbergstrasse 11, 80937, Munich, Germany aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/3(2011-03-01), 193-198 0340-5761 85:3<193 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer