caa a22 4500 445853409 CHVBK 20180317145420.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00204-011-0661-x doi (NATIONALLICENCE)springer-10.1007/s00204-011-0661-x Sub-chronic effect of perfluorooctanesulfonate (PFOS) on the balance of type 1 and type 2 cytokine in adult C57BL6 mice [Elektronische Daten] [Guang-Hui Dong, Miao-Miao Liu, Da Wang, Li Zheng, Zai-Fu Liang, Yi-He Jin] As a ubiquitous and highly persistent environmental contaminant, the clear mechanisms to explain any perfluorooctanesulfonate (PFOS)-induced immunotoxicity are still unknown. This study here sought to examine the ability of PFOS to potentially perturb T-helper (TH)-1 and TH-2 cell cytokine secreting activities, as well as to cause shifts in antibody isotype levels, and possible mechanisms involved in PFOS-induced immunotoxicity. Adult male C57BL/6 mice were exposed to PFOS daily via gavage for 60days [0, 0.5, 1, 5, 25, or 50mg/kg total administered dose (TAD)]. One day after the final exposure, the ex vivo production of the TH1-type cytokines (IL-2 and IFN-γ), TH2-type (IL-4), and IL-10 cytokines by isolated splenocytes, serum levels of immunoglobulin (Ig) were assessed via ELISA or ELISPOT. The results showed that IL-4 secretion was increased at exposure ≥5mg PFOS/kg TAD in a dose-dependent manner. PFOS exposure increased IL-10 but decreased IL-2 and IFN-γ formation markedly at 50mg PFOS/kg TAD. Serum levels of sheep red blood cells (SRBC)-specific IgM synthesis decreased significantly with PFOS exposure in a dose-related manner; serum SRBC-specific IgG, IgG1, and IgE levels increased with 50mg PFOS/kg TAD regimens. These results indicated that, after a long-term exposure to PFOS, a host's immune state is likely to be characterized by a shift toward a more TH2-like state that, in turn, may lead to enhancement of their humoral response and suppression of their cellular response at levels of upper range for occupationally exposed workers or approximately 150-fold for general human population. Springer-Verlag, 2011 Perfluorooctanesulfonate nationallicence Type 1 cytokine nationallicence Type 2 cytokine nationallicence Immunoglobulin nationallicence Dong Guang-Hui School of Public Health, China Medical University, North 2nd Road, 110001, Heping Districts, Shenyang, People's Republic of China aut Liu Miao-Miao School of Public Health, China Medical University, North 2nd Road, 110001, Heping Districts, Shenyang, People's Republic of China aut Wang Da School of Public Health, China Medical University, North 2nd Road, 110001, Heping Districts, Shenyang, People's Republic of China aut Zheng Li Department of Immunology, College of Basic Medical Science, China Medical University, 110001, Shenyang, People's Republic of China aut Liang Zai-Fu Department of Immunology, College of Basic Medical Science, China Medical University, 110001, Shenyang, People's Republic of China aut Jin Yi-He School of Public Health, China Medical University, North 2nd Road, 110001, Heping Districts, Shenyang, People's Republic of China aut Archives of Toxicology Springer-Verlag 85/10(2011-10-01), 1235-1244 0340-5761 85:10<1235 2011 85 204 https://doi.org/10.1007/s00204-011-0661-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-011-0661-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dong Guang-Hui School of Public Health, China Medical University, North 2nd Road, 110001, Heping Districts, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Liu Miao-Miao School of Public Health, China Medical University, North 2nd Road, 110001, Heping Districts, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Wang Da School of Public Health, China Medical University, North 2nd Road, 110001, Heping Districts, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Zheng Li Department of Immunology, College of Basic Medical Science, China Medical University, 110001, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Liang Zai-Fu Department of Immunology, College of Basic Medical Science, China Medical University, 110001, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Jin Yi-He School of Public Health, China Medical University, North 2nd Road, 110001, Heping Districts, Shenyang, People's Republic of China aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/10(2011-10-01), 1235-1244 0340-5761 85:10<1235 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer