caa a22 4500 445853433 CHVBK 20180317145420.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00204-011-0660-y doi (NATIONALLICENCE)springer-10.1007/s00204-011-0660-y Developmental exposure to methylmercury and 2,2′,4,4′,5,5′-hexachlorobiphenyl (PCB153) affects cerebral dopamine D1-like and D2-like receptors of weanling and pubertal rats [Elektronische Daten] [Teresa Coccini, Elisa Roda, Anna Castoldi, Diana Poli, Matteo Goldoni, Maria Vettori, Antonio Mutti, Luigi Manzo] MeHg (0.5mg/kg/day) and/or PCB153 (5mg/kg/day) effects, administered orally to rat dams (GD7-PND21), were explored in PND21 and PND36 offspring brain in terms of density (Bmax) and affinity (Kd) of dopamine D1-like (D1-Rs) and D2-like receptors (D2-Rs), by saturation binding studies. D1-Rs decreased density in both cortex and striatum (15-30%) by MeHg and PCB153, either alone or combined, without additivity in PND21 males. Changes disappeared by PND36. In females, only MeHg caused a 15% Bmax decrease in striatum. D2-Rs enhanced density (23-50%) and reduced affinity in cortex to a similar extent by all treatments in both weanling and pubertal males. Affinity was also decreased in females by all types of exposure at both ages, while density was enhanced by PCB153 only in a delayed manner (PND36). No changes were detected in striatum. In MeHg and MeHg+PCB153 pup cortex, Hg concentrations ranged, on PND21, between 0.25 and 0.89 and 0.94-1.40μg/g tissue, respectively, and were 5- to sixfold lower 2weeks later. PCB153 levels, in PCB153±MeHg treated rats, were about 15μg/g tissue (PND21) and 4-8μg/g tissue (PND36). In striatum, the Hg and PCB153 concentrations were similar to those in cortex. Brain kinetics trend also applied to blood PCB153 or Hg levels. Perinatal exposure to MeHg and/or PCB153 affects D1- and D2-Rs in a gender-, time-, and brain area-dependent manner. Combined treatment does not exacerbate the neurochemical effects of the individual compounds. Springer-Verlag, 2011 Development nationallicence Neurotoxicity nationallicence Polychlorobiphenyls nationallicence Food contaminants nationallicence Mixture nationallicence Bw : Body weight nationallicence DA : Dopamine nationallicence D1-Rs : Dopamine D1-like receptors nationallicence D2-Rs : Dopamine D2-like receptors nationallicence DAT : Dopamine transporter nationallicence DOPAC : Dihydroxyphenylacetic acid nationallicence GD : Gestational day nationallicence PCB153 : 2,2′,4,4′,5,5′-Hexachlorobiphenyl nationallicence HVA : Homovanillic acid nationallicence MeHg : Methylmercury nationallicence PCB : Polychlorinated biphenyl nationallicence PND : Postnatal day nationallicence 5-HT : Serotonin/5-hydroxytriptamine nationallicence TH : Tyrosine hydroxylase nationallicence VMAT : Vesicular monoamine transporter nationallicence Coccini Teresa Toxicology Division, Research Center for Occupational and Environmental Toxicology, IRCCS Salvatore Maugeri Foundation, Institute of Pavia, Via Maugeri, 10, 27100, Pavia, Italy aut Roda Elisa Toxicology Division, Department of Internal Medicine and Therapeutics, University of Pavia, Pavia, Italy aut Castoldi Anna Toxicology Division, Research Center for Occupational and Environmental Toxicology, IRCCS Salvatore Maugeri Foundation, Institute of Pavia, Via Maugeri, 10, 27100, Pavia, Italy aut Poli Diana Department of Clinical Medicine, Nephrology and Health Sciences, Laboratory of Industrial Toxicology, University of Parma, Parma, Italy aut Goldoni Matteo Department of Clinical Medicine, Nephrology and Health Sciences, Laboratory of Industrial Toxicology, University of Parma, Parma, Italy aut Vettori Maria Department of Clinical Medicine, Nephrology and Health Sciences, Laboratory of Industrial Toxicology, University of Parma, Parma, Italy aut Mutti Antonio Department of Clinical Medicine, Nephrology and Health Sciences, Laboratory of Industrial Toxicology, University of Parma, Parma, Italy aut Manzo Luigi Toxicology Division, Research Center for Occupational and Environmental Toxicology, IRCCS Salvatore Maugeri Foundation, Institute of Pavia, Via Maugeri, 10, 27100, Pavia, Italy aut Archives of Toxicology Springer-Verlag 85/10(2011-10-01), 1281-1294 0340-5761 85:10<1281 2011 85 204 https://doi.org/10.1007/s00204-011-0660-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-011-0660-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Coccini Teresa Toxicology Division, Research Center for Occupational and Environmental Toxicology, IRCCS Salvatore Maugeri Foundation, Institute of Pavia, Via Maugeri, 10, 27100, Pavia, Italy aut NATIONALLICENCE 700 1- Roda Elisa Toxicology Division, Department of Internal Medicine and Therapeutics, University of Pavia, Pavia, Italy aut NATIONALLICENCE 700 1- Castoldi Anna Toxicology Division, Research Center for Occupational and Environmental Toxicology, IRCCS Salvatore Maugeri Foundation, Institute of Pavia, Via Maugeri, 10, 27100, Pavia, Italy aut NATIONALLICENCE 700 1- Poli Diana Department of Clinical Medicine, Nephrology and Health Sciences, Laboratory of Industrial Toxicology, University of Parma, Parma, Italy aut NATIONALLICENCE 700 1- Goldoni Matteo Department of Clinical Medicine, Nephrology and Health Sciences, Laboratory of Industrial Toxicology, University of Parma, Parma, Italy aut NATIONALLICENCE 700 1- Vettori Maria Department of Clinical Medicine, Nephrology and Health Sciences, Laboratory of Industrial Toxicology, University of Parma, Parma, Italy aut NATIONALLICENCE 700 1- Mutti Antonio Department of Clinical Medicine, Nephrology and Health Sciences, Laboratory of Industrial Toxicology, University of Parma, Parma, Italy aut NATIONALLICENCE 700 1- Manzo Luigi Toxicology Division, Research Center for Occupational and Environmental Toxicology, IRCCS Salvatore Maugeri Foundation, Institute of Pavia, Via Maugeri, 10, 27100, Pavia, Italy aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/10(2011-10-01), 1281-1294 0340-5761 85:10<1281 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer