caa a22 4500 445853441 CHVBK 20180317145420.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s00204-011-0657-6 doi (NATIONALLICENCE)springer-10.1007/s00204-011-0657-6 Absorption and metabolism of the food contaminant 3-chloro-1,2-propanediol (3-MCPD) and its fatty acid esters by human intestinal Caco-2 cells [Elektronische Daten] [Thorsten Buhrke, Rüdiger Weißhaar, Alfonso Lampen] 3-Chloro-1,2-propanediol (3-MCPD) fatty acid esters are formed upon thermal processing of fat-containing foods in the presence of chloride ions. Upon hydrolytic cleavage, these substances could release free 3-MCPD. This compound is toxicologically well characterised and displayed cancerogenic potential in rodent models. Recently, serious contaminations of different food products with 3-MCPD fatty acid esters have been reported. In regard to a risk assessment, the key question is to which degree these 3-MCPD fatty acid esters are hydrolysed in the human gut. Therefore, the aim of the present project was to examine the hydrolysis of 3-MCPD fatty acid esters and the resulting release of free 3-MCPD by using differentiated Caco-2 cells, a cellular in vitro model for the human intestinal barrier. Here, we show that 3-MCPD fatty acid esters at a concentration of 100μM were neither absorbed by the cells nor the esters were transported via a Caco-2 monolayer. 3-MCPD-1-monoesters were hydrolysed in the presence of Caco-2 cells. In contrast, a 3-MCPD-1,2-diester used in this study was obviously absorbed and metabolised by the cells. Free 3-MCPD was not absorbed by the cells, but the substance migrated through a Caco-2 monolayer by paracellular diffusion. From these in vitro studies, we conclude that 3-MCPD-1-monoesters are likely to be hydrolysed in the human intestine, thereby increasing the burden with free 3-MCPD. In contrast, intestinal cells seem to have the capacity to metabolise 3-MCPD diesters, thereby detoxifying the 3-MCPD moiety. Springer-Verlag, 2011 3-MCPD nationallicence 3-MCPD fatty acid ester nationallicence Food contaminant nationallicence Risk assessment nationallicence Caco-2 nationallicence Buhrke Thorsten Department of Food Safety, Federal Institute For Risk Assessment, Thielallee 88-92, 14195, Berlin, Germany aut Weißhaar Rüdiger CVUA Stuttgart, Schaflandstraße 3/2, 70736, Fellbach, Germany aut Lampen Alfonso Department of Food Safety, Federal Institute For Risk Assessment, Thielallee 88-92, 14195, Berlin, Germany aut Archives of Toxicology Springer-Verlag 85/10(2011-10-01), 1201-1208 0340-5761 85:10<1201 2011 85 204 https://doi.org/10.1007/s00204-011-0657-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-011-0657-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Buhrke Thorsten Department of Food Safety, Federal Institute For Risk Assessment, Thielallee 88-92, 14195, Berlin, Germany aut NATIONALLICENCE 700 1- Weißhaar Rüdiger CVUA Stuttgart, Schaflandstraße 3/2, 70736, Fellbach, Germany aut NATIONALLICENCE 700 1- Lampen Alfonso Department of Food Safety, Federal Institute For Risk Assessment, Thielallee 88-92, 14195, Berlin, Germany aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/10(2011-10-01), 1201-1208 0340-5761 85:10<1201 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer