caa a22 4500 445853549 CHVBK 20180317145421.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00204-011-0685-2 doi (NATIONALLICENCE)springer-10.1007/s00204-011-0685-2 Bitumen workers handling mastic versus rolled asphalt in a tunnel: assessment of exposure and biomarkers of irritation and genotoxicity [Elektronische Daten] [Monika Raulf-Heimsoth, Boleslaw Marczynski, Anne Spickenheuer, Beate Pesch, Peter Welge, Reinhold Rühl, Rainer Bramer, Benjamin Kendzia, Evelyn Heinze, Jürgen Angerer, Thomas Brüning] Emission levels of vapours and aerosols of bitumen are different when processing rolled asphalt compared to mastic asphalt, with working temperatures up to 180 and 250°C, respectively. During the Human Bitumen Study, we examined six workers handling rolled asphalt and mastic asphalt in two consecutive weeks at the same construction site in a tunnel. In addition to the determination of exposure to bitumen and polycyclic aromatic hydrocarbons (PAH) during shift, we examined urinary PAH metabolites, irritative and genotoxic effects before and after shift. Median personal shift concentration of vapours and aerosols of bitumen was 1.8(range 0.9-2.4) mg/m3 during the application of rolled asphalt and 7.9(range 4.9-11.9) mg/m3 when mastic asphalt was applied. Area measurement of vapours and aerosols of bitumen revealed higher concentrations than the personal measurements for mastic asphalt (mastic asphalt: 34.9mg/m3; rolled asphalt: 1.8mg/m3). Processing mastic asphalt was associated also with higher PAH concentrations. Urinary 1-hydroxypyrene and the sum of 1-, 2+9-, 3- and 4-hydroxyphenanthrene increased slightly during shift without clear difference between mastic and rolled asphalt application. However, the post-shift urinary PAH-metabolite concentrations did not reflect the different PAH exposure during mastic and rolled asphalt application. Individual workers could be identified by their spirometry results indicating that these data reflect more chronic than acute effects. In most cases, an increase of 8-oxodGuo adducts was observed during shift that was independent of the asphalt application. 8-oxodGuo and (+)-anti-BPDE-DNA adducts were higher than in exposed workers of the Human Bitumen Study independent of the asphalt application. The DNA-strand breaks were considerably higher pre-shift and decreased during shift. In this study, mastic asphalt application led to significantly higher exposure to vapours and aerosols of bitumen, as well as to airborne PAH, compared to rolled asphalt application. Nevertheless, no differences in the excretion of urinary PAH metabolites, lung function impairment and genotoxic markers were detected. However, higher levels of genotoxicity markers on both examination days compared with the results of the Human Bitumen Study may indicate a possible influence of the specific tunnel setting. Springer-Verlag, 2011 Bitumen nationallicence PAH metabolites nationallicence Spirometry nationallicence Genotoxic biomarkers nationallicence Rolled asphalt nationallicence Mastic asphalt nationallicence Raulf-Heimsoth Monika Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut Marczynski Boleslaw Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut Spickenheuer Anne Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut Pesch Beate Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut Welge Peter Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut Rühl Reinhold BG-BAU, An der Festeburg 27-29, Frankfurt/Main, Germany aut Bramer Rainer Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut Kendzia Benjamin Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut Heinze Evelyn Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut Angerer Jürgen Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut Brüning Thomas Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut Archives of Toxicology Springer-Verlag 85(2011-06-01), 81-87 0340-5761 85<81 2011 85 204 https://doi.org/10.1007/s00204-011-0685-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-011-0685-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Raulf-Heimsoth Monika Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut NATIONALLICENCE 700 1- Marczynski Boleslaw Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut NATIONALLICENCE 700 1- Spickenheuer Anne Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut NATIONALLICENCE 700 1- Pesch Beate Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut NATIONALLICENCE 700 1- Welge Peter Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut NATIONALLICENCE 700 1- Rühl Reinhold BG-BAU, An der Festeburg 27-29, Frankfurt/Main, Germany aut NATIONALLICENCE 700 1- Bramer Rainer Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut NATIONALLICENCE 700 1- Kendzia Benjamin Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut NATIONALLICENCE 700 1- Heinze Evelyn Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut NATIONALLICENCE 700 1- Angerer Jürgen Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut NATIONALLICENCE 700 1- Brüning Thomas Institute for Prevention and Occupational Medicine of German Social Accident Insurance, Institute of the Ruhr University Bochum (IPA), Bürkle-de-la-Camp-Platz 1, 44789, Bochum, Germany aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85(2011-06-01), 81-87 0340-5761 85<81 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer