caa a22 4500 445853638 CHVBK 20180317145421.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00204-010-0590-0 doi (NATIONALLICENCE)springer-10.1007/s00204-010-0590-0 Long-term detection of methyltestosterone (ab-) use by a yeast transactivation system [Elektronische Daten] [Sylvi Wolf, Patrick Diel, Maria Parr, Felicitas Rataj, Willhelm Schänzer, Günter Vollmer, Oliver Zierau] The routinely used analytical method for detecting the abuse of anabolic steroids only allows the detection of molecules with known analytical properties. In our supplementary approach to structure-independent detection, substances are identified by their biological activity. In the present study, urines excreted after oral methyltestosterone (MT) administration were analyzed by a yeast androgen screen (YAS). The aim was to trace the excretion of MT or its metabolites in human urine samples and to compare the results with those from the established analytical method. MT and its two major metabolites were tested as pure compounds in the YAS. In a second step, the ability of the YAS to detect MT and its metabolites in urine samples was analyzed. For this purpose, a human volunteer ingested of a single dose of 5mg methyltestosterone. Urine samples were collected after different time intervals (0-307h) and were analyzed in the YAS and in parallel by GC/MS. Whereas the YAS was able to trace MT in urine samples at least for 14days, the detection limits of the GC/MS method allowed follow-up until day six. In conclusion, our results demonstrate that the yeast reporter gene system could detect the activity of anabolic steroids like methyltestosterone with high sensitivity even in urine. Furthermore, the YAS was able to detect MT abuse for a longer period of time than classical GC/MS. Obviously, the system responds to long-lasting metabolites yet unidentified. Therefore, the YAS can be a powerful (pre-) screening tool with the potential that to be used to identify persistent or late screening metabolites of anabolic steroids, which could be used for an enhancement of the sensitivity of GC/MS detection techniques. Springer-Verlag, 2010 Methyltestosterone nationallicence 17β-Hydroxy-17α-methylandrost-4-ene-3-one, 17α-methyl-5α-androstane-3α,17β-diol nationallicence 17α-methyl-5β-androstane-3α,17β-diol nationallicence Urine nationallicence Reporter gene system nationallicence Doping nationallicence Wolf Sylvi Molecular Cell Physiology and Endocrinology, Institute of Zoology, Technical University Dresden, Zellescher Weg 20b, Room 248, 01217, Dresden, Germany aut Diel Patrick Department of Molecular and Cellular Sports Medicine, Center for Preventive Doping Research, Institute of Cardiovascular Research and Sports Medicine, German Sport University Cologne, Cologne, Germany aut Parr Maria Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Cologne, Germany aut Rataj Felicitas Molecular Cell Physiology and Endocrinology, Institute of Zoology, Technical University Dresden, Zellescher Weg 20b, Room 248, 01217, Dresden, Germany aut Schänzer Willhelm Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Cologne, Germany aut Vollmer Günter Molecular Cell Physiology and Endocrinology, Institute of Zoology, Technical University Dresden, Zellescher Weg 20b, Room 248, 01217, Dresden, Germany aut Zierau Oliver Molecular Cell Physiology and Endocrinology, Institute of Zoology, Technical University Dresden, Zellescher Weg 20b, Room 248, 01217, Dresden, Germany aut Archives of Toxicology Springer-Verlag 85/4(2011-04-01), 285-292 0340-5761 85:4<285 2011 85 204 https://doi.org/10.1007/s00204-010-0590-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0590-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wolf Sylvi Molecular Cell Physiology and Endocrinology, Institute of Zoology, Technical University Dresden, Zellescher Weg 20b, Room 248, 01217, Dresden, Germany aut NATIONALLICENCE 700 1- Diel Patrick Department of Molecular and Cellular Sports Medicine, Center for Preventive Doping Research, Institute of Cardiovascular Research and Sports Medicine, German Sport University Cologne, Cologne, Germany aut NATIONALLICENCE 700 1- Parr Maria Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Cologne, Germany aut NATIONALLICENCE 700 1- Rataj Felicitas Molecular Cell Physiology and Endocrinology, Institute of Zoology, Technical University Dresden, Zellescher Weg 20b, Room 248, 01217, Dresden, Germany aut NATIONALLICENCE 700 1- Schänzer Willhelm Center for Preventive Doping Research, Institute of Biochemistry, German Sport University Cologne, Cologne, Germany aut NATIONALLICENCE 700 1- Vollmer Günter Molecular Cell Physiology and Endocrinology, Institute of Zoology, Technical University Dresden, Zellescher Weg 20b, Room 248, 01217, Dresden, Germany aut NATIONALLICENCE 700 1- Zierau Oliver Molecular Cell Physiology and Endocrinology, Institute of Zoology, Technical University Dresden, Zellescher Weg 20b, Room 248, 01217, Dresden, Germany aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/4(2011-04-01), 285-292 0340-5761 85:4<285 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer