caa a22 4500 445853662 CHVBK 20180317145421.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00204-010-0591-z doi (NATIONALLICENCE)springer-10.1007/s00204-010-0591-z Ethanol-induced small heat shock protein genes in the differentiation of mouse embryonic neural stem cells [Elektronische Daten] [Mi Choi, Kyoung Jung, Ji Park, Nando Das, Mi Chung, Ihn Choi, Boung Lee, Kyoung Park, Young Chai] Neural stem cells (NSCs) of the neuroepithelium differentiate into one of three central nervous system (CNS) cell lineages: neurons, astrocytes, or oligodendrocytes. In this study, the differentiation potential of NSCs from the forebrain of embryonic day 15 (E15) mouse embryos was analyzed using immunocytochemistry. NSCs were differentiated early in the presence or absence of ethanol (50mM), and gene expression patterns among NSCs, differentiated cells and ethanol-treated differentiated cells were assessed by microarray and real-time PCR analysis. Genes that were up-regulated in differentiated cells both in the presence and in the absence of ethanol when compared to NSCs were related to the Wnt signaling pathway, including Ctnna1, Wnt5a, Wnt5b, Wnt7a, Fzd3, and Fzd2; genes related to cell adhesion, including Cadm1, Ncam1, and Ncam2; and genes encoding small heat shock proteins, including HspB2, HspB7, and HspB8. In particular, the expression levels of HspB2 and HspB7 were elevated in ethanol-treated differentiated cells compared to non-treated differentiated cells. The gene expression patterns of various heat shock transcription factors (HSFs), proteins that regulate the transcription of heat shock genes, were also analyzed. The expression levels of HSF2 and HSF5 increased in differentiated cells in the presence and absence of ethanol when compared to NSCs. Of these two genes, HSF5 demonstrated an enhanced up-regulation, particularly in ethanol-treated differentiated cells compared to cells that were differentiated in the absence of ethanol. These results imply that HspB2 and HspB7, which are small heat shock proteins with tissue-restricted expression profiles, might be up-regulated by ethanol during the short-term differentiation of NSCs. Springer-Verlag, 2010 Differentiation nationallicence Ethanol nationallicence Heat shock transcription factor nationallicence Microarray nationallicence Neural stem cells nationallicence Small heat shock protein nationallicence Choi Mi Division of Molecular and Life Sciences, Hanyang University, 426-791, Ansan, Republic of Korea aut Jung Kyoung Division of Molecular and Life Sciences, Hanyang University, 426-791, Ansan, Republic of Korea aut Park Ji Division of Molecular and Life Sciences, Hanyang University, 426-791, Ansan, Republic of Korea aut Das Nando Division of Molecular and Life Sciences, Hanyang University, 426-791, Ansan, Republic of Korea aut Chung Mi Fertility Center of CHA General Hospital, CHA Research Institute, 135-080, Seoul, Korea aut Choi Ihn Department of Neuropsychiatry, College of Medicine, Hallym University, 150-719, Seoul, Korea aut Lee Boung Department of Neuropsychiatry, College of Medicine, Hallym University, 150-719, Seoul, Korea aut Park Kyoung Department of Physiology, College of Medicine, Seoul National University, 110-799, Seoul, Korea aut Chai Young Division of Molecular and Life Sciences, Hanyang University, 426-791, Ansan, Republic of Korea aut Archives of Toxicology Springer-Verlag 85/4(2011-04-01), 293-304 0340-5761 85:4<293 2011 85 204 https://doi.org/10.1007/s00204-010-0591-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0591-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Choi Mi Division of Molecular and Life Sciences, Hanyang University, 426-791, Ansan, Republic of Korea aut NATIONALLICENCE 700 1- Jung Kyoung Division of Molecular and Life Sciences, Hanyang University, 426-791, Ansan, Republic of Korea aut NATIONALLICENCE 700 1- Park Ji Division of Molecular and Life Sciences, Hanyang University, 426-791, Ansan, Republic of Korea aut NATIONALLICENCE 700 1- Das Nando Division of Molecular and Life Sciences, Hanyang University, 426-791, Ansan, Republic of Korea aut NATIONALLICENCE 700 1- Chung Mi Fertility Center of CHA General Hospital, CHA Research Institute, 135-080, Seoul, Korea aut NATIONALLICENCE 700 1- Choi Ihn Department of Neuropsychiatry, College of Medicine, Hallym University, 150-719, Seoul, Korea aut NATIONALLICENCE 700 1- Lee Boung Department of Neuropsychiatry, College of Medicine, Hallym University, 150-719, Seoul, Korea aut NATIONALLICENCE 700 1- Park Kyoung Department of Physiology, College of Medicine, Seoul National University, 110-799, Seoul, Korea aut NATIONALLICENCE 700 1- Chai Young Division of Molecular and Life Sciences, Hanyang University, 426-791, Ansan, Republic of Korea aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/4(2011-04-01), 293-304 0340-5761 85:4<293 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer