caa a22 4500 445853840 CHVBK 20180317145422.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00204-010-0619-4 doi (NATIONALLICENCE)springer-10.1007/s00204-010-0619-4 Combined effect of fluoride and 2,3,7,8-tetrachlorodibenzo- p -dioxin on mouse dental hard tissue formation in vitro [Elektronische Daten] [Eija Salmela, Pirjo-Liisa Lukinmaa, Anna-Maija Partanen, Carin Sahlberg, Satu Alaluusua] Fluoride interferes with enamel matrix secretion and mineralization and dentin mineralization. The most toxic dioxin congener, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), also impairs dental hard tissue formation and mineralization in vitro and in vivo. Our aim was to investigate in vitro whether the combined effect of sodium fluoride (NaF) and TCDD on dental hard tissue formation is potentiative. For this purpose, mandibular first and second molar tooth germs of E18 mouse embryos were cultured for 5-12days with NaF and TCDD alone at various concentrations (2.5, 5, 10, 12.5, 15, and 20μM and 5, 10, 12.5, and 15nM, respectively) to determine the highest concentrations, which alone cause no or negligible effects. Morphological changes were studied from the whole tooth photographs and histological tissue sections. The concentrations found were 15μM for NaF and 10nM for TCDD. While at these concentrations, the effects of NaF and TCDD alone were barely detectable, the effect of simultaneous exposure on dentin and enamel formation was overt; mineralization of predentin to dentin and enamel matrix secretion and mineralization were impaired. Immunohistochemical analysis revealed that the combined exposure modified amelogenin expression by odontoblasts. Morphology of ameloblasts and the expression of amelogenin indicated that ameloblasts were still secretory. The results show that NaF and TCDD have potentiative, harmful effects on the formation of dental hard tissues. Since children can be exposed to subclinical levels of fluoride and dioxins during early childhood, coincidently with mineralization of the first permanent teeth, this finding may have clinical significance. Springer-Verlag, 2010 Amelogenesis nationallicence Dentinogenesis nationallicence Fluoride nationallicence TCDD nationallicence Mouse nationallicence Salmela Eija Department of Pediatric and Preventive Dentistry, Institute of Dentistry, University of Helsinki, P.O. Box 41, 00014, Helsinki, Finland aut Lukinmaa Pirjo-Liisa Department of Oral Pathology, Institute of Dentistry, University of Helsinki, P.O. Box 41, 00014, Helsinki, Finland aut Partanen Anna-Maija Department of Pediatric and Preventive Dentistry, Institute of Dentistry, University of Helsinki, P.O. Box 41, 00014, Helsinki, Finland aut Sahlberg Carin Department of Pediatric and Preventive Dentistry, Institute of Dentistry, University of Helsinki, P.O. Box 41, 00014, Helsinki, Finland aut Alaluusua Satu Department of Pediatric and Preventive Dentistry, Institute of Dentistry, University of Helsinki, P.O. Box 41, 00014, Helsinki, Finland aut Archives of Toxicology Springer-Verlag 85/8(2011-08-01), 953-963 0340-5761 85:8<953 2011 85 204 https://doi.org/10.1007/s00204-010-0619-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0619-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Salmela Eija Department of Pediatric and Preventive Dentistry, Institute of Dentistry, University of Helsinki, P.O. Box 41, 00014, Helsinki, Finland aut NATIONALLICENCE 700 1- Lukinmaa Pirjo-Liisa Department of Oral Pathology, Institute of Dentistry, University of Helsinki, P.O. Box 41, 00014, Helsinki, Finland aut NATIONALLICENCE 700 1- Partanen Anna-Maija Department of Pediatric and Preventive Dentistry, Institute of Dentistry, University of Helsinki, P.O. Box 41, 00014, Helsinki, Finland aut NATIONALLICENCE 700 1- Sahlberg Carin Department of Pediatric and Preventive Dentistry, Institute of Dentistry, University of Helsinki, P.O. Box 41, 00014, Helsinki, Finland aut NATIONALLICENCE 700 1- Alaluusua Satu Department of Pediatric and Preventive Dentistry, Institute of Dentistry, University of Helsinki, P.O. Box 41, 00014, Helsinki, Finland aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/8(2011-08-01), 953-963 0340-5761 85:8<953 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer