caa a22 4500 445853859 CHVBK 20180317145422.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00204-010-0623-8 doi (NATIONALLICENCE)springer-10.1007/s00204-010-0623-8 Effect of methylmercury administration on choroid plexus function in rats [Elektronische Daten] [Masaaki Nakamura, Akira Yasutake, Masatake Fujimura, Noriyuki Hachiya, Masumi Marumoto] Methylmercury (MeHg) is a well-known environmental neurotoxin. The choroid plexus (CP), the main component of the blood-cerebrospinal fluid (CSF) barrier (BCSFB), protects the brain from xenobiotics, similar to the blood-brain barrier. Because CP is considered a critical target site of MeHg-induced neurotoxic damage, functional alterations in CP may be caused in relation to the extent of MeHg-induced brain injury. To test this hypothesis, we examined time-dependent pathological alterations in rats administered subtoxic (asymptomatic group) or toxic (symptomatic group) MeHg doses for 3weeks after the cessation of MeHg administration. We primarily assessed (1) mercury concentrations in the brain, CSF, and plasma; (2) histopathological changes in the brain; (3) albumin CSF/plasma concentration quotient (Qalb), an index of BCSFB dysfunction; and (4) concentration of CSF transthyretin (TTR), which is primarily produced in CP. Mercury concentrations in the brain, CSF, and plasma decreased, and Qalb and CSF TTR concentrations did not change significantly in the asymptomatic group. In the symptomatic group, brain and CSF mercury concentrations did not decrease for 2weeks after the cessation of MeHg administration, but no pathological alteration occurred in the brain during this period. Pathological changes in the cerebellum became evident 3weeks after the cessation of MeHg administration. Furthermore, Qalb continued to increase after the cessation of MeHg administration, whereas no decrease in CSF TTR concentration was observed, indicating selective impairment of CP function. These findings suggest that MeHg at toxic doses causes selective functional alteration of CP before leading to pathological alterations in the brain. Springer-Verlag, 2010 Methylmercury nationallicence Choroid plexus nationallicence Cerebrospinal fluid nationallicence Qalb nationallicence Transthyretin nationallicence BBB : blood-brain barrier nationallicence BCSFB : blood-CSF barrier nationallicence CNS : central nervous system nationallicence CP : choroid plexus nationallicence CSF : cerebrospinal fluid nationallicence Ct : threshold cycles nationallicence GFAP : glial fibrillary acidic protein nationallicence MeHg : methylmercury nationallicence MUSTag : Multiple Simultaneous Tag nationallicence Qalb : CSF/plasma concentration quotient nationallicence RT-PCR : reverse transcriptase-polymerase chain reaction nationallicence SELDI-TOF-MS : surface-enhanced laser desorption ionization-time-of-flight-mass spectrometry nationallicence TTR : transthyretin nationallicence TUNEL : terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling nationallicence Nakamura Masaaki Department of Clinical Medicine, National Institute for Minamata Disease, 4058-18 Hama, 867-0008, Minamata, Japan aut Yasutake Akira Department of Basic Medical Sciences, National Institute for Minamata Disease, 4058-18 Hama, 867-0008, Minamata, Japan aut Fujimura Masatake Department of Basic Medical Sciences, National Institute for Minamata Disease, 4058-18 Hama, 867-0008, Minamata, Japan aut Hachiya Noriyuki Department of International Affairs and Environmental Sciences, National Institute for Minamata Disease, 4058-18 Hama, 867-0008, Minamata, Japan aut Marumoto Masumi Department of Basic Medical Sciences, National Institute for Minamata Disease, 4058-18 Hama, 867-0008, Minamata, Japan aut Archives of Toxicology Springer-Verlag 85/8(2011-08-01), 911-918 0340-5761 85:8<911 2011 85 204 https://doi.org/10.1007/s00204-010-0623-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0623-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakamura Masaaki Department of Clinical Medicine, National Institute for Minamata Disease, 4058-18 Hama, 867-0008, Minamata, Japan aut NATIONALLICENCE 700 1- Yasutake Akira Department of Basic Medical Sciences, National Institute for Minamata Disease, 4058-18 Hama, 867-0008, Minamata, Japan aut NATIONALLICENCE 700 1- Fujimura Masatake Department of Basic Medical Sciences, National Institute for Minamata Disease, 4058-18 Hama, 867-0008, Minamata, Japan aut NATIONALLICENCE 700 1- Hachiya Noriyuki Department of International Affairs and Environmental Sciences, National Institute for Minamata Disease, 4058-18 Hama, 867-0008, Minamata, Japan aut NATIONALLICENCE 700 1- Marumoto Masumi Department of Basic Medical Sciences, National Institute for Minamata Disease, 4058-18 Hama, 867-0008, Minamata, Japan aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/8(2011-08-01), 911-918 0340-5761 85:8<911 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer