caa a22 4500 445853883 CHVBK 20180317145422.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00204-010-0622-9 doi (NATIONALLICENCE)springer-10.1007/s00204-010-0622-9 Disruptive neuronal development by acrylamide in the hippocampal dentate hilus after developmental exposure in rats [Elektronische Daten] [Bunichiro Ogawa, Takumi Ohishi, Liyun Wang, Miwa Takahashi, Eriko Taniai, Hitomi Hayashi, Kunitoshi Mitsumori, Makoto Shibutani] To examine whether developmental exposure to acrylamide (AA) impairs neuronal development, pregnant Sprague-Dawley rats were treated with AA at 0, 25, 50 or 100ppm in drinking water from gestational day 6 until weaning on postnatal day 21. Offspring were immunohistochemically examined at the end of exposure. We investigated the expression of Reelin (a molecule regulating neuronal migration and positioning) in the hilus of the hippocampal dentate gyrus. As a positive control for direct exposure, AA (50mg/kg body weight) was administered to pups by intraperitoneal injection 3times per week during the lactation period. As well as pups directly injected with AA, maternally exposed offspring decreased body weight at 100ppm; increased dose-dependently the number of Reelin-immunoreactive cells (from 25ppm AA) and glutamic acid decarboxylase 67-immunoreactive cells (from 50ppm AA), confirming an increase in γ-aminobutyric acid-ergic interneurons. We also noted decreased apoptosis in the neuroblast-producing subgranular zone of the dentate gyrus of maternally exposed pups at 100ppm, as well as in directly AA-injected pups. These results suggest that a compensatory regulatory mechanism exists to correct impaired neurogenesis and mismigration caused by maternal exposure to AA during neuronal development. The lowest-observed-adverse-effect level of AA was determined to be 25ppm (3.72mg/kg body weight/day). Springer-Verlag, 2010 Acrylamide nationallicence Developmental neurotoxicity nationallicence Neuronal migration nationallicence Neurogenesis nationallicence Dentate gyrus nationallicence GABAergic interneuron nationallicence AA : Acrylamide nationallicence CA1 : Cornu ammonis 1 nationallicence CA2 : Cornu ammonis 2 nationallicence CA3 : Cornu ammonis 3 nationallicence Calb-D-28K : Calbindin-D-28K nationallicence GABA : γ-aminobutyric acid nationallicence GAD67 : Glutamic acid decarboxylase 67 nationallicence GD : Gestational day nationallicence PCNA : Proliferating cell nuclear antigen nationallicence PND : Postnatal day nationallicence Ogawa Bunichiro Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, 183-8509, Tokyo, Japan aut Ohishi Takumi Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, 183-8509, Tokyo, Japan aut Wang Liyun Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, 183-8509, Tokyo, Japan aut Takahashi Miwa Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, 158-8501, Tokyo, Japan aut Taniai Eriko Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, 183-8509, Tokyo, Japan aut Hayashi Hitomi Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, 183-8509, Tokyo, Japan aut Mitsumori Kunitoshi Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, 183-8509, Tokyo, Japan aut Shibutani Makoto Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, 183-8509, Tokyo, Japan aut Archives of Toxicology Springer-Verlag 85/8(2011-08-01), 987-994 0340-5761 85:8<987 2011 85 204 https://doi.org/10.1007/s00204-010-0622-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0622-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ogawa Bunichiro Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, 183-8509, Tokyo, Japan aut NATIONALLICENCE 700 1- Ohishi Takumi Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, 183-8509, Tokyo, Japan aut NATIONALLICENCE 700 1- Wang Liyun Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, 183-8509, Tokyo, Japan aut NATIONALLICENCE 700 1- Takahashi Miwa Division of Pathology, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, 158-8501, Tokyo, Japan aut NATIONALLICENCE 700 1- Taniai Eriko Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, 183-8509, Tokyo, Japan aut NATIONALLICENCE 700 1- Hayashi Hitomi Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, 183-8509, Tokyo, Japan aut NATIONALLICENCE 700 1- Mitsumori Kunitoshi Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, 183-8509, Tokyo, Japan aut NATIONALLICENCE 700 1- Shibutani Makoto Laboratory of Veterinary Pathology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, 183-8509, Tokyo, Japan aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/8(2011-08-01), 987-994 0340-5761 85:8<987 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer