caa a22 4500 445853972 CHVBK 20180317145422.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00204-010-0558-0 doi (NATIONALLICENCE)springer-10.1007/s00204-010-0558-0 Induction of DNA strand breaks by dental composite components compared to X-ray exposure in human gingival fibroblasts [Elektronische Daten] [Jürgen Durner, Małgorzata Dębiak, Alexander Bürkle, Reinhard Hickel, Franz-Xaver Reichl] The toxicity of dental composites has been attributed to the release of residual monomers from polymerized resin-based composites due to degradation processes or incomplete polymerization. Some of these eluted substances have a genotoxic potential. We tested the hypothesis that realistic concentrations (and/or worst case concentrations/situations) of bisphenol-A-glycidyldimethacrylate (BisGMA), triethyleneglycol dimethacrylate (TEGDMA), 2-hydroxyethyl methacrylate (HEMA) and methyl methacrylate (MMA) found in elution experiments can cause DNA strand breaks in human gingival fibroblasts (HGF). Such DNA damage was compared with that resulting from ionizing radiation coming from natural sources, dental radiography or tumor therapy. TEGDMA, HEMA and MMA did not induce DNA strand breaks at concentrations of up to 10mM. About 24h after incubation with 0.25mM BisGMA, significantly more DNA strand breaks were found in HGF compared to controls. DNA strand breaks caused by 0.25mM BisGMA, correspond to DNA strand breakage caused by irradiation with 4Gy, only used in the high single-dose irradiation tumor therapy. But 0.25mM BisGMA is more than 100-fold higher than that concentration found in worst case calculations. Therefore, our data did not support our hypothesis. Springer-Verlag, 2010 TEGDMA nationallicence HEMA nationallicence DNA-strand breaks nationallicence X-ray nationallicence Genotoxic potential nationallicence Durner Jürgen Department of Operative/Restorative Dentistry, Periodontology and Pedodontics, Ludwig-Maximilians-University of Munich, Goethestrasse 70, 80336, Munich, Germany aut Dębiak Małgorzata Department of Biology, Molecular Toxicology Group, University of Konstanz, Jacob-Burckhardt-Str. 31, 78464, Constance, Germany aut Bürkle Alexander Department of Biology, Molecular Toxicology Group, University of Konstanz, Jacob-Burckhardt-Str. 31, 78464, Constance, Germany aut Hickel Reinhard Department of Operative/Restorative Dentistry, Periodontology and Pedodontics, Ludwig-Maximilians-University of Munich, Goethestrasse 70, 80336, Munich, Germany aut Reichl Franz-Xaver Department of Operative/Restorative Dentistry, Periodontology and Pedodontics, Ludwig-Maximilians-University of Munich, Goethestrasse 70, 80336, Munich, Germany aut Archives of Toxicology Springer-Verlag 85/2(2011-02-01), 143-148 0340-5761 85:2<143 2011 85 204 https://doi.org/10.1007/s00204-010-0558-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0558-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Durner Jürgen Department of Operative/Restorative Dentistry, Periodontology and Pedodontics, Ludwig-Maximilians-University of Munich, Goethestrasse 70, 80336, Munich, Germany aut NATIONALLICENCE 700 1- Dębiak Małgorzata Department of Biology, Molecular Toxicology Group, University of Konstanz, Jacob-Burckhardt-Str. 31, 78464, Constance, Germany aut NATIONALLICENCE 700 1- Bürkle Alexander Department of Biology, Molecular Toxicology Group, University of Konstanz, Jacob-Burckhardt-Str. 31, 78464, Constance, Germany aut NATIONALLICENCE 700 1- Hickel Reinhard Department of Operative/Restorative Dentistry, Periodontology and Pedodontics, Ludwig-Maximilians-University of Munich, Goethestrasse 70, 80336, Munich, Germany aut NATIONALLICENCE 700 1- Reichl Franz-Xaver Department of Operative/Restorative Dentistry, Periodontology and Pedodontics, Ludwig-Maximilians-University of Munich, Goethestrasse 70, 80336, Munich, Germany aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/2(2011-02-01), 143-148 0340-5761 85:2<143 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer