caa a22 4500 445854006 CHVBK 20180317145422.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00204-010-0563-3 doi (NATIONALLICENCE)springer-10.1007/s00204-010-0563-3 Determination of 2,5-toluylenediamine (2,5-TDA) and aromatic amines in urine after personal application of hair dyes: kinetics and doses [Elektronische Daten] [Thomas Schettgen, K. Heinrich, T. Kraus, Monika Gube] The personal use of hair dye products is currently under discussion due to the potentially increased risk of bladder cancer among long-time users described in epidemiological literature. In order to investigate the dermal absorption of aromatic diamines as well as aromatic amines possibly present as contaminants in hair dye formulations, we conducted a biomonitoring study under real-life conditions and calculated kinetics and doses for the urinary excretion. Urine samples of two female subjects were collected for a time period of 48h after personal application of a hair dye cream and analysed for aromatic diamines as well as o-toluidine and 4-aminobiphenyl using highly specific GC/MS-methods. 2,5-Toluylenediamine (2,5-TDA) as active ingredient of hair dyes is rapidly absorbed dermally. After a distribution phase of 12h, 2,5-TDA is excreted with a half-time of 8h. Excretion was 90% complete within 24h after application. The doses of 2,5-TDA excreted within 48h were 700μg for application of a brown-reddish hair dye cream and 1.5mg for the application of a brown-black hair dye cream. Urinary 4-aminobiphenyl as well as contaminations with other aromatic diamines were not detectable in our study. Due to the artifactual formation of o-toluidine in the presence of high concentrations of urinary 2,5-TDA, our results could not prove an increased internal exposure of humans to carcinogenic amines after personal application of hair dyes. Springer-Verlag, 2010 Hair dye nationallicence Aromatic diamines nationallicence Aromatic amines nationallicence Biological monitoring nationallicence o -Toluidine nationallicence Urine nationallicence Toxicokinetics nationallicence Schettgen Thomas Institute for Occupational and Social Medicine, Medical Faculty, RWTH Aachen University, Pauwelsstrasse 30, 52074, Aachen, Germany aut Heinrich K. Institute for Occupational and Social Medicine, Medical Faculty, RWTH Aachen University, Pauwelsstrasse 30, 52074, Aachen, Germany aut Kraus T. Institute for Occupational and Social Medicine, Medical Faculty, RWTH Aachen University, Pauwelsstrasse 30, 52074, Aachen, Germany aut Gube Monika Institute for Occupational and Social Medicine, Medical Faculty, RWTH Aachen University, Pauwelsstrasse 30, 52074, Aachen, Germany aut Archives of Toxicology Springer-Verlag 85/2(2011-02-01), 127-133 0340-5761 85:2<127 2011 85 204 https://doi.org/10.1007/s00204-010-0563-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0563-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schettgen Thomas Institute for Occupational and Social Medicine, Medical Faculty, RWTH Aachen University, Pauwelsstrasse 30, 52074, Aachen, Germany aut NATIONALLICENCE 700 1- Heinrich K. Institute for Occupational and Social Medicine, Medical Faculty, RWTH Aachen University, Pauwelsstrasse 30, 52074, Aachen, Germany aut NATIONALLICENCE 700 1- Kraus T. Institute for Occupational and Social Medicine, Medical Faculty, RWTH Aachen University, Pauwelsstrasse 30, 52074, Aachen, Germany aut NATIONALLICENCE 700 1- Gube Monika Institute for Occupational and Social Medicine, Medical Faculty, RWTH Aachen University, Pauwelsstrasse 30, 52074, Aachen, Germany aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/2(2011-02-01), 127-133 0340-5761 85:2<127 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer