caa a22 4500 445854014 CHVBK 20180317145422.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s00204-010-0556-2 doi (NATIONALLICENCE)springer-10.1007/s00204-010-0556-2 Effects of extracellular acidic-alkaline stresses on trigeminal ganglion neurons in the mouse embryo in vivo [Elektronische Daten] [Shingo Mukai, Hiroshi Nakagawa, Hiroyuki Ichikawa, Shuichi Miyazaki, Kazuhiko Nishimura, Saburo Matsuo] Acidic-alkaline stresses caused by ischemia and hypoglycemia induce neuronal cell death resulting from intracellular pH disturbance. The effects of acidic-alkaline disturbance on the trigeminal ganglion (TG) neurons of the embryonic mouse were investigated by caspase-3-immunohistochemistry and Nissl staining. TG neurons exhibited apoptosis in 3.08±0.55% of neurons in intact embryos at day 16. Intraperitoneal injection of alkaline solution (pH 8.97; 0.005-0.1M K2HPO4 or 0.01-0.04M KOH) into the embryo at embryonic day 15 significantly increased the number of apoptotic neurons in the TG at embryonic day 16 with dependence on concentration (3.40-6.05 and 2.93-5.55%, respectively). On the other hand, acidic solutions (pH 4.4; 0.01-0.2M KH2PO4) slightly, but not significantly, increased the number of apoptotic cells (3.64-5.15%, without dependence on concentration). Neutral solutions (pH 7.4; 0.01-0.2M potassium phosphate buffer) had no effect on neuronal survival in the TG (2.89-3.48%). The results indicated that alkaline stress significantly increased apoptosis in the developing nervous system, but acidic stress did not. Springer-Verlag, 2010 Cell death nationallicence Excitotoxicity nationallicence Extracellular ions nationallicence Neurotoxicity nationallicence Embryogenesis nationallicence Mukai Shingo Laboratory of Toxicology, Course of Veterinary Science, Graduate School of Life and Environmental Biosciences, Osaka Prefecture University, 1-58, Rinku-ourai-kita, 598-8531, Izumisano, Japan aut Nakagawa Hiroshi Laboratory of Toxicology, Course of Veterinary Science, Graduate School of Life and Environmental Biosciences, Osaka Prefecture University, 1-58, Rinku-ourai-kita, 598-8531, Izumisano, Japan aut Ichikawa Hiroyuki Division of Oral and Craniofacial Anatomy, Tohoku University Graduate School of Dentistry, 980-8575, Sendai, Japan aut Miyazaki Shuichi Department of Microbiology and Infections Diseases, Toho University Faculty of Medicine, 143-8540, Tokyo, Japan aut Nishimura Kazuhiko Laboratory of Toxicology, Course of Veterinary Science, Graduate School of Life and Environmental Biosciences, Osaka Prefecture University, 1-58, Rinku-ourai-kita, 598-8531, Izumisano, Japan aut Matsuo Saburo Laboratory of Toxicology, Course of Veterinary Science, Graduate School of Life and Environmental Biosciences, Osaka Prefecture University, 1-58, Rinku-ourai-kita, 598-8531, Izumisano, Japan aut Archives of Toxicology Springer-Verlag 85/2(2011-02-01), 149-154 0340-5761 85:2<149 2011 85 204 https://doi.org/10.1007/s00204-010-0556-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0556-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mukai Shingo Laboratory of Toxicology, Course of Veterinary Science, Graduate School of Life and Environmental Biosciences, Osaka Prefecture University, 1-58, Rinku-ourai-kita, 598-8531, Izumisano, Japan aut NATIONALLICENCE 700 1- Nakagawa Hiroshi Laboratory of Toxicology, Course of Veterinary Science, Graduate School of Life and Environmental Biosciences, Osaka Prefecture University, 1-58, Rinku-ourai-kita, 598-8531, Izumisano, Japan aut NATIONALLICENCE 700 1- Ichikawa Hiroyuki Division of Oral and Craniofacial Anatomy, Tohoku University Graduate School of Dentistry, 980-8575, Sendai, Japan aut NATIONALLICENCE 700 1- Miyazaki Shuichi Department of Microbiology and Infections Diseases, Toho University Faculty of Medicine, 143-8540, Tokyo, Japan aut NATIONALLICENCE 700 1- Nishimura Kazuhiko Laboratory of Toxicology, Course of Veterinary Science, Graduate School of Life and Environmental Biosciences, Osaka Prefecture University, 1-58, Rinku-ourai-kita, 598-8531, Izumisano, Japan aut NATIONALLICENCE 700 1- Matsuo Saburo Laboratory of Toxicology, Course of Veterinary Science, Graduate School of Life and Environmental Biosciences, Osaka Prefecture University, 1-58, Rinku-ourai-kita, 598-8531, Izumisano, Japan aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/2(2011-02-01), 149-154 0340-5761 85:2<149 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer