caa a22 4500 445854111 CHVBK 20180317145423.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00204-011-0673-6 doi (NATIONALLICENCE)springer-10.1007/s00204-011-0673-6 Depletion of CD4+CD25+Foxp3+ regulatory T cells with anti-CD25 antibody may exacerbate the 1,3-β-glucan-induced lung inflammatory response in mice [Elektronische Daten] [Fangwei Liu, Dong Weng, Ying Chen, Laiyu Song, Cuiying Li, Lei Dong, Yuan Wang, Shasha Tao, Jie Chen] 1,3-β-Glucan was a major cell wall component of fungus. The existing studies showed that 1,3-β-glucan exposure could induce lung inflammation that involved both Th1 and Th2 cytokines. Regulatory T cells (Treg cells) played a critical role in regulating immune homeostasis by adjusting the Th1/Th2 balance. The role of Treg cells and regulatory mechanism in 1,3-β-glucan-induced lung inflammation is still unclear. In our study, mice were exposed to 1,3-β-glucan by intratracheal instillation. To investigate the role of Treg cells in response to 1,3-β-glucan, we generated Treg-depleted mice by intraperitoneal administration of anti-CD25 mAb. The Treg-depleted mice showed more inflammatory cells and severer pathological inflammatory change in lung tissue. Depletion of Treg cells led to increased Th1 cytokines and decreased Th2 cytokines. Treg-depleted mice showed a decreased expression of anti-inflammation cytokine and lower-level expression of CTLA-4. In all, our study indicated that Treg cells participated in regulating the 1,3-β-glucan-induced lung inflammation. Depletion of Treg cells aggravated the 1,3-β-glucan-induced lung inflammation, regulated the Th1/Th2 balance by enhancing Th1 response. Treg cells exerted their modulation function depending on both direct and indirect mechanism during the 1,3-β-glucan-induced lung inflammation. Springer-Verlag, 2011 1,3-β-glucan nationallicence Regulatory T cells nationallicence Lung inflammation nationallicence Th1/Th2 nationallicence Liu Fangwei Division of Pneumoconiosis, School of Public Health, China Medical University, 110001, Shenyang, Liaoning, People's Republic of China aut Weng Dong Division of Pneumoconiosis, School of Public Health, China Medical University, 110001, Shenyang, Liaoning, People's Republic of China aut Chen Ying Division of Pneumoconiosis, School of Public Health, China Medical University, 110001, Shenyang, Liaoning, People's Republic of China aut Song Laiyu Division of Pneumoconiosis, School of Public Health, China Medical University, 110001, Shenyang, Liaoning, People's Republic of China aut Li Cuiying Division of Pneumoconiosis, School of Public Health, China Medical University, 110001, Shenyang, Liaoning, People's Republic of China aut Dong Lei Division of Pneumoconiosis, School of Public Health, China Medical University, 110001, Shenyang, Liaoning, People's Republic of China aut Wang Yuan Division of Pneumoconiosis, School of Public Health, China Medical University, 110001, Shenyang, Liaoning, People's Republic of China aut Tao Shasha Division of Pneumoconiosis, School of Public Health, China Medical University, 110001, Shenyang, Liaoning, People's Republic of China aut Chen Jie Division of Pneumoconiosis, School of Public Health, China Medical University, 110001, Shenyang, Liaoning, People's Republic of China aut Archives of Toxicology Springer-Verlag 85/11(2011-11-01), 1383-1394 0340-5761 85:11<1383 2011 85 204 https://doi.org/10.1007/s00204-011-0673-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-011-0673-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Fangwei Division of Pneumoconiosis, School of Public Health, China Medical University, 110001, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Weng Dong Division of Pneumoconiosis, School of Public Health, China Medical University, 110001, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Chen Ying Division of Pneumoconiosis, School of Public Health, China Medical University, 110001, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Song Laiyu Division of Pneumoconiosis, School of Public Health, China Medical University, 110001, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Li Cuiying Division of Pneumoconiosis, School of Public Health, China Medical University, 110001, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Dong Lei Division of Pneumoconiosis, School of Public Health, China Medical University, 110001, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Wang Yuan Division of Pneumoconiosis, School of Public Health, China Medical University, 110001, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Tao Shasha Division of Pneumoconiosis, School of Public Health, China Medical University, 110001, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 700 1- Chen Jie Division of Pneumoconiosis, School of Public Health, China Medical University, 110001, Shenyang, Liaoning, People's Republic of China aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/11(2011-11-01), 1383-1394 0340-5761 85:11<1383 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer