caa a22 4500 44585412X CHVBK 20180317145423.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00204-010-0593-x doi (NATIONALLICENCE)springer-10.1007/s00204-010-0593-x Bisphenol A-glycidyl methacrylate induces a broad spectrum of DNA damage in human lymphocytes [Elektronische Daten] [Kinga Drozdz, Daniel Wysokinski, Renata Krupa, Katarzyna Wozniak] Bisphenol A-glycidyl methacrylate (BisGMA) is monomer of dental filling composites, which can be released from these materials and cause adverse biologic effects in human cells. In the present work, we investigated genotoxic effect of BisGMA on human lymphocytes and human acute lymphoblastic leukemia cell line (CCRF-CEM) cells. Our results indicate that BisGMA is genotoxic for human lymphocytes. The compound induced DNA damage evaluated by the alkaline, neutral, and pH 12.1 version of the comet assay. This damage included oxidative modifications of the DNA bases, as checked by DNA repair enzymes EndoIII and Fpg, alkali-labile sites and DNA double-strand breaks. BisGMA induced DNA-strand breaks in the isolated plasmid. Lymphocytes incubated with BisGMA at 1mM were able to remove about 50% of DNA damage during 120-min repair incubation. The monomer at 1mM evoked a delay of the cell cycle in the S phase in CCRF-CEM cells. The experiment with spin trap—DMPO demonstrated that BisGMA induced reactive oxygen species, which were able to damage DNA. BisGMA is able to induce a broad spectrum of DNA damage including severe DNA double-strand breaks, which can be responsible for a delay of the cell cycle in the S phase. The Author(s), 2010 BisGMA nationallicence DNA damage nationallicence DNA repair nationallicence Reactive oxygen species nationallicence Cell cycle nationallicence Drozdz Kinga Department of Molecular Genetics, University of Lodz, Lodz, Poland aut Wysokinski Daniel Department of Molecular Genetics, University of Lodz, Lodz, Poland aut Krupa Renata Laboratory of DNA Repair, Department of Molecular Genetics, University of Lodz, Lodz, Poland aut Wozniak Katarzyna Laboratory of DNA Repair, Department of Molecular Genetics, University of Lodz, Lodz, Poland aut Archives of Toxicology Springer-Verlag 85/11(2011-11-01), 1453-1461 0340-5761 85:11<1453 2011 85 204 https://doi.org/10.1007/s00204-010-0593-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-010-0593-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Drozdz Kinga Department of Molecular Genetics, University of Lodz, Lodz, Poland aut NATIONALLICENCE 700 1- Wysokinski Daniel Department of Molecular Genetics, University of Lodz, Lodz, Poland aut NATIONALLICENCE 700 1- Krupa Renata Laboratory of DNA Repair, Department of Molecular Genetics, University of Lodz, Lodz, Poland aut NATIONALLICENCE 700 1- Wozniak Katarzyna Laboratory of DNA Repair, Department of Molecular Genetics, University of Lodz, Lodz, Poland aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/11(2011-11-01), 1453-1461 0340-5761 85:11<1453 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer