caa a22 4500 445854154 CHVBK 20180317145423.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00204-011-0697-y doi (NATIONALLICENCE)springer-10.1007/s00204-011-0697-y Sodium fluoride suppress proliferation and induce apoptosis through decreased insulin-like growth factor-I expression and oxidative stress in primary cultured mouse osteoblasts [Elektronische Daten] [Zhi Wang, Xiaoyu Yang, Shiyong Yang, Gaixian Ren, Miro Ferreri, Yong Su, Liben Chen, Bo Han] It has been reported that sodium fluoride suppressed proliferation and induced apoptosis in osteoblasts. However, the details about the mechanism at work in bone metabolism are limited. In this study, we further investigated the mechanisms of NaF on proliferation and apoptosis in the primary cultured mouse osteoblasts, which were exposed to different concentration of NaF (10−6-5×10−4M). We examined the effect of NaF on proliferation, cell cycle, apoptosis, oxidative stress, and the protein level of insulin-like growth factor-I (IGF-I) in osteoblasts. All the tested NaF inhibited proliferation and arrested cell cycle at S phase in osteoblasts, and further demonstrated to induce apoptosis in osteoblasts. On the other hand, we found that NaF increased oxidative stress and decreased protein expression of IGF-I. Our study herein suggested that NaF caused proliferation suppression, and apoptosis may contribute to decrease IGF-I expression and increased oxidative stress damage by NaF in the primary mouse osteoblasts. Springer-Verlag, 2011 Sodium fluoride nationallicence Proliferation nationallicence Apoptosis nationallicence Insulin-like growth factor-I nationallicence Oxidative stress nationallicence Wang Zhi College of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193, Beijing, China aut Yang Xiaoyu Department of Orthopaedics, China-Japan Union Hospital, Jilin University, 130033, Changchun, China aut Yang Shiyong College of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193, Beijing, China aut Ren Gaixian College of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193, Beijing, China aut Ferreri Miro College of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193, Beijing, China aut Su Yong College of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193, Beijing, China aut Chen Liben College of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193, Beijing, China aut Han Bo College of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193, Beijing, China aut Archives of Toxicology Springer-Verlag 85/11(2011-11-01), 1407-1417 0340-5761 85:11<1407 2011 85 204 https://doi.org/10.1007/s00204-011-0697-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-011-0697-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Zhi College of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193, Beijing, China aut NATIONALLICENCE 700 1- Yang Xiaoyu Department of Orthopaedics, China-Japan Union Hospital, Jilin University, 130033, Changchun, China aut NATIONALLICENCE 700 1- Yang Shiyong College of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193, Beijing, China aut NATIONALLICENCE 700 1- Ren Gaixian College of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193, Beijing, China aut NATIONALLICENCE 700 1- Ferreri Miro College of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193, Beijing, China aut NATIONALLICENCE 700 1- Su Yong College of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193, Beijing, China aut NATIONALLICENCE 700 1- Chen Liben College of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193, Beijing, China aut NATIONALLICENCE 700 1- Han Bo College of Veterinary Medicine, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193, Beijing, China aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/11(2011-11-01), 1407-1417 0340-5761 85:11<1407 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer