caa a22 4500 445854162 CHVBK 20180317145423.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00204-011-0672-7 doi (NATIONALLICENCE)springer-10.1007/s00204-011-0672-7 Fluoride-induced apoptosis and gene expression profiling in mice sperm in vivo [Elektronische Daten] [Zilong Sun, Ruiyan Niu, Bin Wang, Zhibin Jiao, Jinming Wang, Jianhai Zhang, Shaolin Wang, Jundong Wang] Exposure to fluoride can induce low sperm quality; however, little is known about the molecular mechanisms by which fluoride exerts its toxic effects. This study was conducted to evaluate ultrastructure, oxidative stress, and apoptosis in sperm of mice treated with 150mg/l NaF for 49days. Furthermore, microarray analysis was also utilized to characterize the effects of fluoride in gene expression profiling on mice sperm. An increased ROS and a decreased TAC accompanied with distinct morphological changes and significant apoptosis were observed in mice sperm from the fluoride group. Fluoride exposure also significantly elevated the protein expressions of cytochrome c and active caspase-3. In global gene expression profiling, 34 up-regulated and 63 down-regulated genes, which are involved in several sperm biological processes including signal transduction, oxidative stress, apoptosis, electron transport, glycolysis, chemotaxis, spermatogenesis, and sperm capacitation, were significantly differentially expressed. Based on these findings, it was proposed that oxidative stress induced by excessive ROS may trigger sperm apoptosis through mitochondrial impairment, resulting in decreased fertility in mice exposed to fluoride. Microarray analysis also provided several important biological clues for further investigating fluoride-induced damage in sperm morphology and functions. Springer-Verlag, 2011 Fluoride nationallicence Mice nationallicence Sperm nationallicence Apoptosis nationallicence Gene expression profiling nationallicence Sun Zilong Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, People's Republic of China aut Niu Ruiyan Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, People's Republic of China aut Wang Bin Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, People's Republic of China aut Jiao Zhibin Center for Molecular Systems Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut Wang Jinming Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, People's Republic of China aut Zhang Jianhai Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, People's Republic of China aut Wang Shaolin Department of Psychiatry and Neurobehavioral Sciences, University of Virginia, 22911, Charlottesville, VA, USA aut Wang Jundong Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, People's Republic of China aut Archives of Toxicology Springer-Verlag 85/11(2011-11-01), 1441-1452 0340-5761 85:11<1441 2011 85 204 https://doi.org/10.1007/s00204-011-0672-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00204-011-0672-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sun Zilong Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, People's Republic of China aut NATIONALLICENCE 700 1- Niu Ruiyan Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, People's Republic of China aut NATIONALLICENCE 700 1- Wang Bin Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, People's Republic of China aut NATIONALLICENCE 700 1- Jiao Zhibin Center for Molecular Systems Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wang Jinming Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Jianhai Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, People's Republic of China aut NATIONALLICENCE 700 1- Wang Shaolin Department of Psychiatry and Neurobehavioral Sciences, University of Virginia, 22911, Charlottesville, VA, USA aut NATIONALLICENCE 700 1- Wang Jundong Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, 030801, Shanxi, People's Republic of China aut NATIONALLICENCE 773 0- Archives of Toxicology Springer-Verlag 85/11(2011-11-01), 1441-1452 0340-5761 85:11<1441 2011 85 204 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer