caa a22 4500 445856858 CHVBK 20180317145430.0 cr unu---uuuuu 170323e20110201xx s 000 0 eng 10.1007/s10592-010-0142-4 doi (NATIONALLICENCE)springer-10.1007/s10592-010-0142-4 Conservation genetics of Butte County meadowfoam ( Limnanthes floccosa ssp. californica Arroyo), an endangered vernal pool endemic [Elektronische Daten] [Christina Sloop, Carolina Pickens, Sarah Gordon] The endangered annual plant Limnanthes floccosa ssp. californica Arroyo is restricted to vernal pools in Butte County, California. To identify populations with unique genetic resources, guide reintroduction efforts, and design seed collection scenarios for long-term ex situ seed storage we determined extant genetic diversity and structure by surveying 457 individuals from 21 distinct populations using nine polymorphic microsatellite markers. We found low within population genetic diversity: low allelic diversity (1.9 [0.06 SE] alleles/locus); low heterozygosity (H obs=0.10±0.018, H exp=0.19±0.015), and a high fixation index (0.556±0.044). The number of polymorphic loci ranged between 11 and 89%. Bayesian ordination determined 20 distinct populations and we found high genetic structure among these (F st=0.65, P<0.0001). We identified notable gene flow barriers across populations, confirming regional structuring between three previously defined population density centers and two outlying populations (F st=0.21, P<0.0001). Population size estimates ranged between ~50 and >5,000 extant plants per site. Our study confirms previous isozyme-based results and suggests that the loss of any population would represent a significant loss in the species' genetic diversity. Recovery requires active restoration of existing populations and permanent habitat protection. We recommend close comparison of microhabitats of declining populations with genetically similar populations, to determine the potential for human assisted gene flow via seed movement to recover declining populations. Springer Science+Business Media B.V., 2010 Genetic structure nationallicence Genetic diversity nationallicence Vernal pool plants nationallicence Endangered species nationallicence Self-fertilization nationallicence Limnanthes sp nationallicence Sloop Christina San Francisco Bay Joint Venture, 735 B Center Blvd., 94930, Fairfax, CA, USA aut Pickens Carolina Department of Biology, Sonoma State University, 1801 E. Cotati Ave, 94928, Rohnert Park, CA, USA aut Gordon Sarah Department of Biology, Sonoma State University, 1801 E. Cotati Ave, 94928, Rohnert Park, CA, USA aut Conservation Genetics Springer Netherlands 12/1(2011-02-01), 311-323 1566-0621 12:1<311 2011 12 10592 https://doi.org/10.1007/s10592-010-0142-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10592-010-0142-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sloop Christina San Francisco Bay Joint Venture, 735 B Center Blvd., 94930, Fairfax, CA, USA aut NATIONALLICENCE 700 1- Pickens Carolina Department of Biology, Sonoma State University, 1801 E. Cotati Ave, 94928, Rohnert Park, CA, USA aut NATIONALLICENCE 700 1- Gordon Sarah Department of Biology, Sonoma State University, 1801 E. Cotati Ave, 94928, Rohnert Park, CA, USA aut NATIONALLICENCE 773 0- Conservation Genetics Springer Netherlands 12/1(2011-02-01), 311-323 1566-0621 12:1<311 2011 12 10592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer