caa a22 4500 445857188 CHVBK 20180317145431.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10592-011-0243-8 doi (NATIONALLICENCE)springer-10.1007/s10592-011-0243-8 Genetic diversity and population structure of pumas ( Puma concolor ) in southeastern Brazil: implications for conservation in a human-dominated landscape [Elektronische Daten] [R. Miotto, M. Cervini, M. Figueiredo, R. Begotti, P. Galetti Jr.] São Paulo is the most populous, developed and industrialized state of Brazil. Despite of intensive human activities, large habitat loss and fragmentation of the native vegetation cover, pumas (Puma concolor) still inhabit remnant habitat fragments in the northeastern area of the state. We investigated the occurrence of genetic structure and levels of genetic variability on pumas to aggregate basic information for conservation efforts to maintain long term viable populations of this top-predator in the region. By analyzing microsatellite loci variation, we corroborated the hypothesis of absence of genetic structure, and estimated high levels of genetic diversity (expected heterozygosity of 0.79 and mean of 10 alleles per locus). In spite of the increasing number of roadkilling and puma-human conflicts in the area, apparently pumas still maintain some level of gene flow between protected areas of the region. The observed excess of heterozygotes suggests a recent bottleneck event in this population, probably a consequence of the profound landscape transformation of the studied area during the last century; another possibility is this may be due to the observed deviation in the population sex ratio, which may be influencing the pumas' mating system. We propose that: (1) landscape management in the study area should be focused on increasing habitat connectivity, creating protected areas and structures to allow highway crossing of pumas; (2) educational actions should be undertaken to change community perception of large carnivores, and possibly the implementation of compensatory actions to ranchers. Springer Science+Business Media B.V., 2011 Fecal DNA nationallicence Genetic variability nationallicence Landscape management nationallicence Microsatellite nationallicence Noninvasive analysis nationallicence Protected areas nationallicence Miotto R. Laboratório de Biodiversidade Molecular e Conservação, Departamento de Genética e Evolução, Universidade Federal de São Carlos, UFSCar, Rodovia Washington Luis, km 235, Monjolinho, 13565-905, São Carlos, SP, Brazil aut Cervini M. Laboratório de Genética Molecular, Departamento de Ciências Biológicas, Universidade Estadual do Sudoeste da Bahia, UESB, Jequié, BA, Brazil aut Figueiredo M. Departamento de Zootecnia, Universidade Estadual Paulista ‘Julio de Mesquita Filho' UNESP, Jaboticabal, SP, Brazil aut Begotti R. Departamento de Ciências Florestais, Escola Superior de Agricultura ‘Luiz de Queiroz', ESALQ/USP, Piracicaba, SP, Brazil aut Galetti Jr. P. Laboratório de Biodiversidade Molecular e Conservação, Departamento de Genética e Evolução, Universidade Federal de São Carlos, UFSCar, Rodovia Washington Luis, km 235, Monjolinho, 13565-905, São Carlos, SP, Brazil aut Conservation Genetics Springer Netherlands 12/6(2011-12-01), 1447-1455 1566-0621 12:6<1447 2011 12 10592 https://doi.org/10.1007/s10592-011-0243-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10592-011-0243-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miotto R. Laboratório de Biodiversidade Molecular e Conservação, Departamento de Genética e Evolução, Universidade Federal de São Carlos, UFSCar, Rodovia Washington Luis, km 235, Monjolinho, 13565-905, São Carlos, SP, Brazil aut NATIONALLICENCE 700 1- Cervini M. Laboratório de Genética Molecular, Departamento de Ciências Biológicas, Universidade Estadual do Sudoeste da Bahia, UESB, Jequié, BA, Brazil aut NATIONALLICENCE 700 1- Figueiredo M. Departamento de Zootecnia, Universidade Estadual Paulista ‘Julio de Mesquita Filho' UNESP, Jaboticabal, SP, Brazil aut NATIONALLICENCE 700 1- Begotti R. Departamento de Ciências Florestais, Escola Superior de Agricultura ‘Luiz de Queiroz', ESALQ/USP, Piracicaba, SP, Brazil aut NATIONALLICENCE 700 1- Galetti Jr P. Laboratório de Biodiversidade Molecular e Conservação, Departamento de Genética e Evolução, Universidade Federal de São Carlos, UFSCar, Rodovia Washington Luis, km 235, Monjolinho, 13565-905, São Carlos, SP, Brazil aut NATIONALLICENCE 773 0- Conservation Genetics Springer Netherlands 12/6(2011-12-01), 1447-1455 1566-0621 12:6<1447 2011 12 10592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer