caa a22 4500 445857250 CHVBK 20180317145431.0 cr unu---uuuuu 170323e20111201xx s 000 0 eng 10.1007/s10592-011-0245-6 doi (NATIONALLICENCE)springer-10.1007/s10592-011-0245-6 Genetic structure in remnant populations of an endangered cyprinodontid fish, Orestias ascotanensis , endemic to the Ascotán salt pan of the Altiplano [Elektronische Daten] [Pamela Morales, Irma Vila, Elie Poulin] The impact of recent habitat fragmentation on population genetic diversity and structure has often been studied, mainly related to anthropogenic causes; however its long-term effect has been much less evaluated. In this study we analyzed the genetic variability of Orestias ascotanensis, a fish endemic to the Ascotán salt pan of Chile. This species, which formed a single and large population during the last wet period that ended 10,000years ago, is currently represented by small populations inhabiting freshwater springs on the eastern border of the salt pan. Therefore, this species represents a unique model to evaluate the consequences of a drastic habitat fragmentation process that initiated thousands of years ago. Analysis of the control region of the mitochondrial DNA revealed high genetic diversity (haplotipic diversity ranged between 0.78 and 0.94) and marked differences among populations (ΦST=0.46). Estimated effective population sizes greatly surpassed the real sizes, particularly among springs that remained connected. These results reflect the long-term consequences of habitat fragmentation on natural populations: structuring of the populations and loss of genetic diversity of the isolated fragments. Springer Science+Business Media B.V., 2011 Genetic drift nationallicence mtDNA Control region nationallicence Habitat fragmentation nationallicence Effective population size nationallicence Freshwater fish nationallicence Morales Pamela Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Ñuñoa, Casilla 653, Santiago, Chile aut Vila Irma Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Ñuñoa, Casilla 653, Santiago, Chile aut Poulin Elie Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Ñuñoa, Casilla 653, Santiago, Chile aut Conservation Genetics Springer Netherlands 12/6(2011-12-01), 1639-1643 1566-0621 12:6<1639 2011 12 10592 https://doi.org/10.1007/s10592-011-0245-6 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10592-011-0245-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morales Pamela Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Ñuñoa, Casilla 653, Santiago, Chile aut NATIONALLICENCE 700 1- Vila Irma Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Ñuñoa, Casilla 653, Santiago, Chile aut NATIONALLICENCE 700 1- Poulin Elie Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Ñuñoa, Casilla 653, Santiago, Chile aut NATIONALLICENCE 773 0- Conservation Genetics Springer Netherlands 12/6(2011-12-01), 1639-1643 1566-0621 12:6<1639 2011 12 10592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer