caa a22 4500 445857374 CHVBK 20180317145432.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10592-010-0179-4 doi (NATIONALLICENCE)springer-10.1007/s10592-010-0179-4 Substantial genetic structure among stocked and native populations of the European grayling ( Thymallus thymallus , Salmonidae) in the United Kingdom [Elektronische Daten] [Nick Dawnay, Louise Dawnay, Roger Hughes, Richard Cove, Martin Taylor] While currently in a state of recovery in the United Kingdom (UK), the grayling (Thymallus thymallus) remains of conservation interest due to its historical decline, socio-economic value and the potential impact of hatchery-reared stock fish on the genetic structure and diversity of wild populations. However, little is known about the levels and distribution of genetic diversity among UK grayling populations. To this end, 27 UK populations of grayling were genotyped across 10 microsatellite loci and sequenced at the mtDNA D-Loop. All populations clustered into four higher-level groups: Northern England, Southern England, Wales, and group consisting of a mixture of native and introduced populations. Ten populations showed evidence of bottleneck or founder effects, and the effective population size (Ne) was low in all populations. In most cases, historical stocking records agreed with the genetic relationships revealed in the study. A D-Loop haplotype network supported the groupings observed in the nuclear data, while phylogenetic inference places the UK populations amongst Central European samples. The combined datasets demonstrate that many of the UK populations can be treated as separate Management Units and we recommend that to preserve population specific genetic diversity, that stocking should be an intervention of last resort. However, if stocking is deemed essential, brood stock should originate from the river to be stocked. Springer Science+Business Media B.V., 2011 Conservation nationallicence Population structure nationallicence Genetic nationallicence Bottleneck nationallicence Stocking nationallicence Ne nationallicence Thymallus nationallicence Dawnay Nick Molecular Ecology and Fisheries Genetics Laboratory, School of Biological Sciences, Bangor University, ECW Building, Bangor, LL57 2UW, Gwynedd, UK aut Dawnay Louise Molecular Ecology and Fisheries Genetics Laboratory, School of Biological Sciences, Bangor University, ECW Building, Bangor, LL57 2UW, Gwynedd, UK aut Hughes Roger Molecular Ecology and Fisheries Genetics Laboratory, School of Biological Sciences, Bangor University, ECW Building, Bangor, LL57 2UW, Gwynedd, UK aut Cove Richard Environment Agency, Chester Road Buckley, CH7 3AJ, Flintshire, UK aut Taylor Martin Molecular Ecology and Fisheries Genetics Laboratory, School of Biological Sciences, Bangor University, ECW Building, Bangor, LL57 2UW, Gwynedd, UK aut Conservation Genetics Springer Netherlands 12/3(2011-06-01), 731-744 1566-0621 12:3<731 2011 12 10592 https://doi.org/10.1007/s10592-010-0179-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10592-010-0179-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dawnay Nick Molecular Ecology and Fisheries Genetics Laboratory, School of Biological Sciences, Bangor University, ECW Building, Bangor, LL57 2UW, Gwynedd, UK aut NATIONALLICENCE 700 1- Dawnay Louise Molecular Ecology and Fisheries Genetics Laboratory, School of Biological Sciences, Bangor University, ECW Building, Bangor, LL57 2UW, Gwynedd, UK aut NATIONALLICENCE 700 1- Hughes Roger Molecular Ecology and Fisheries Genetics Laboratory, School of Biological Sciences, Bangor University, ECW Building, Bangor, LL57 2UW, Gwynedd, UK aut NATIONALLICENCE 700 1- Cove Richard Environment Agency, Chester Road Buckley, CH7 3AJ, Flintshire, UK aut NATIONALLICENCE 700 1- Taylor Martin Molecular Ecology and Fisheries Genetics Laboratory, School of Biological Sciences, Bangor University, ECW Building, Bangor, LL57 2UW, Gwynedd, UK aut NATIONALLICENCE 773 0- Conservation Genetics Springer Netherlands 12/3(2011-06-01), 731-744 1566-0621 12:3<731 2011 12 10592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer