caa a22 4500 445857498 CHVBK 20180317145432.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10592-010-0174-9 doi (NATIONALLICENCE)springer-10.1007/s10592-010-0174-9 Relatedness and polyandry of sixgill sharks, Hexanchus griseus , in an urban estuary [Elektronische Daten] [Shawn Larson, Jeff Christiansen, Denise Griffing, Jimiane Ashe, Dayv Lowry, Kelly Andrews] The bluntnose sixgill shark (Hexanchus griseus) is a widely distributed species found in tropical and temperate waters of every ocean, yet we know relatively little about their basic biology including their life history and population structure. From 2003-2007, we collected over 300 biopsy samples from sixgills during research operations in Puget Sound, WA, USA. Genotypic data using ten polymorphic microsatellites were used to describe sixgill genetic diversity, relatedness and mating pattern. Diversity within sixgills was found to be moderate with an average observed heterozygosity of 0.45, an average expected heterozygosity of 0.61, an average of 12 alleles per locus, and an average allelic richness of eight within microsatellite loci. Our data suggests all of the sampled individuals come from one intermixing population, and we found no historical evidence of significant population bottlenecks. Many of the sharks were sampled using longline techniques with several sharks captured at the same time and place. Similarly, multiple sharks were sampled on several occasions during research events at the Seattle Aquarium. The proportion of individuals that were full- or half-siblings was high among sharks sampled at the same time and place (range 0.65-0.87). Analysis of the genetic relationship between one large female washed ashore and 71 of her near-term pups suggested a polyandrous mating system with as many as nine males contributing to her offspring. This study is the first to investigate genetic diversity, relatedness and paternity within sixgill sharks and sheds light on important conservation implications for this little known shark population. The Author(s), 2010 Bluntnose sixgill nationallicence Hexanchus griseus nationallicence Microsatellites nationallicence Relatedness nationallicence Polyandry nationallicence Shark nationallicence Larson Shawn Seattle Aquarium, 1483 Alaskan Way, Pier 59, 98101-2059, Seattle, WA, USA aut Christiansen Jeff Seattle Aquarium, 1483 Alaskan Way, Pier 59, 98101-2059, Seattle, WA, USA aut Griffing Denise Seattle Aquarium, 1483 Alaskan Way, Pier 59, 98101-2059, Seattle, WA, USA aut Ashe Jimiane Seattle Aquarium, 1483 Alaskan Way, Pier 59, 98101-2059, Seattle, WA, USA aut Lowry Dayv Washington Department of Fish and Wildlife, 1111 Washington Street SE, 98501, Olympia, WA, USA aut Andrews Kelly NOAA Fisheries, Northwest Fisheries Science Center, 2725 Montlake Boulevard East, 98112, Seattle, WA, USA aut Conservation Genetics Springer Netherlands 12/3(2011-06-01), 679-690 1566-0621 12:3<679 2011 12 10592 https://doi.org/10.1007/s10592-010-0174-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10592-010-0174-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Larson Shawn Seattle Aquarium, 1483 Alaskan Way, Pier 59, 98101-2059, Seattle, WA, USA aut NATIONALLICENCE 700 1- Christiansen Jeff Seattle Aquarium, 1483 Alaskan Way, Pier 59, 98101-2059, Seattle, WA, USA aut NATIONALLICENCE 700 1- Griffing Denise Seattle Aquarium, 1483 Alaskan Way, Pier 59, 98101-2059, Seattle, WA, USA aut NATIONALLICENCE 700 1- Ashe Jimiane Seattle Aquarium, 1483 Alaskan Way, Pier 59, 98101-2059, Seattle, WA, USA aut NATIONALLICENCE 700 1- Lowry Dayv Washington Department of Fish and Wildlife, 1111 Washington Street SE, 98501, Olympia, WA, USA aut NATIONALLICENCE 700 1- Andrews Kelly NOAA Fisheries, Northwest Fisheries Science Center, 2725 Montlake Boulevard East, 98112, Seattle, WA, USA aut NATIONALLICENCE 773 0- Conservation Genetics Springer Netherlands 12/3(2011-06-01), 679-690 1566-0621 12:3<679 2011 12 10592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer