caa a22 4500 445857501 CHVBK 20180317145432.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10592-010-0177-6 doi (NATIONALLICENCE)springer-10.1007/s10592-010-0177-6 Signatures of demographic bottlenecks in European wolf populations [Elektronische Daten] [Natalia Sastre, Carles Vilà, María Salinas, Vladimir Bologov, Vicente Urios, Armand Sánchez, Olga Francino, Oscar Ramírez] Monitoring the loss of genetic diversity in wild populations after a bottleneck event is a priority in conservation and management plans. Here, we used diverse molecular markers to search for signatures of demographic bottlenecks in two wolf populations; an isolated population from the Iberian Peninsula and a non-isolated population from European Russia. Autosomal, mtDNA and Y-chromosomal diversity and the effective population size (Ne) were significantly lower in the Iberian population. Neutrality tests using mtDNA sequences, such as R2, Fu and Li's F*, Tajima's D and Fu's Fs, were positively significant in the Iberian population, suggesting a population decline, but were not significant for the Russian population, likely due to its larger effective population size. However, three tests using autosomal data confirmed the occurrence of the genetic bottleneck in both populations. The M-ratio test was the only one providing significant results for both populations. Given the lack of consistency among the different tests, we recommend using multiple approaches to investigate possible past bottlenecks. The small effective population size (about 50) in the Iberian Peninsula compared to the presumed extant population size could indicate that the bottleneck was more powerful than initially suspected or an overestimation of the current population. The risks associated with small effective population sizes suggest that the genetic change in this population should be closely monitored in the future. On the other hand, the relatively small effective population size for Russian wolves (a few hundred individuals) could indicate some fragmentation, contrary to what is commonly assumed. Springer Science+Business Media B.V., 2010 Canis lupus nationallicence mtDNA nationallicence Neutrality nationallicence Y-chromosome nationallicence Autosomal microsatellites nationallicence Effective population size nationallicence European wolf nationallicence Sastre Natalia Servei Veterinari de Genètica Molecular. Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain aut Vilà Carles Estación Biológica de Doñana-CSIC, Avd Américo Vespucio s/n, 41092, Sevilla, Spain aut Salinas María Servei Veterinari de Genètica Molecular. Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain aut Bologov Vladimir Central Forest State Natural Biosphere Reserve, P/o Zapovednik, Nelidovskij rajon, 172513, Tverskaja Oblast, Russia aut Urios Vicente Centro Iberoamericano de la Biodiversidad (CIBIO), Universidad de Alicante, 03080, Alicante, Spain aut Sánchez Armand Servei Veterinari de Genètica Molecular. Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain aut Francino Olga Servei Veterinari de Genètica Molecular. Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain aut Ramírez Oscar Servei Veterinari de Genètica Molecular. Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain aut Conservation Genetics Springer Netherlands 12/3(2011-06-01), 701-712 1566-0621 12:3<701 2011 12 10592 https://doi.org/10.1007/s10592-010-0177-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10592-010-0177-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sastre Natalia Servei Veterinari de Genètica Molecular. Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain aut NATIONALLICENCE 700 1- Vilà Carles Estación Biológica de Doñana-CSIC, Avd Américo Vespucio s/n, 41092, Sevilla, Spain aut NATIONALLICENCE 700 1- Salinas María Servei Veterinari de Genètica Molecular. Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain aut NATIONALLICENCE 700 1- Bologov Vladimir Central Forest State Natural Biosphere Reserve, P/o Zapovednik, Nelidovskij rajon, 172513, Tverskaja Oblast, Russia aut NATIONALLICENCE 700 1- Urios Vicente Centro Iberoamericano de la Biodiversidad (CIBIO), Universidad de Alicante, 03080, Alicante, Spain aut NATIONALLICENCE 700 1- Sánchez Armand Servei Veterinari de Genètica Molecular. Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain aut NATIONALLICENCE 700 1- Francino Olga Servei Veterinari de Genètica Molecular. Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain aut NATIONALLICENCE 700 1- Ramírez Oscar Servei Veterinari de Genètica Molecular. Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain aut NATIONALLICENCE 773 0- Conservation Genetics Springer Netherlands 12/3(2011-06-01), 701-712 1566-0621 12:3<701 2011 12 10592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer