caa a22 4500 445857544 CHVBK 20180317145432.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10592-011-0184-2 doi (NATIONALLICENCE)springer-10.1007/s10592-011-0184-2 Using microsatellite diversity in wild Anegada iguanas ( Cyclura pinguis ) to establish relatedness in a captive breeding group of this critically endangered species [Elektronische Daten] [Adele Mitchell, Jennie Lau, Leona Chemnick, Elizabeth Thompson, Allison Alberts, Oliver Ryder, Glenn Gerber] Awareness of the genealogical relationships between founder animals in captive breeding programs is essential for the selection of mating pairs that maintain genetic diversity. If captive founder relationships are unknown they can be inferred using genetic data from wild populations. Here, we report the results of such an analysis for six Cyclura pinguis (Sauria: Iguanidae) acquired as adults in 1999 by the San Diego Zoo Institute for Conservation Research to begin a captive breeding program for this critically endangered species. The six founder animals were reportedly hatched in captivity from eggs collected on Anegada in 1985. No records exist, however, as to where on Anegada the eggs were collected or from how many nests they originated. To assist determination of genealogical relationships, we genotyped the six captive founders, their first six offspring, and 33 wild adult iguanas from Anegada at 23 informative microsatellite loci. With these data, we estimated allele frequencies among the wild samples and then estimated the relatedness of the captive population. Using likelihood inference, we determined that three closely related pairs exist among the six captive founders and that each pair is not closely related to the other two. In addition, we were able to assign parentage for all six of the founders' offspring tested, one of which had been previously misdiagnosed. Using the assigned parentage and inferred relatedness of the six founders, we calculated mean kinship for each of the six founders and their five living offspring. Finally, based on the allelic diversity of the wild iguanas sampled, we conclude that the C. pinguis population on Anegada is not excessively inbred; however, further investigation is warranted. Springer Science+Business Media B.V., 2011 Genetic variation nationallicence British Virgin Islands nationallicence Greater Antilles nationallicence Caribbean nationallicence Lizard nationallicence Likelihood inference nationallicence Mitchell Adele Department of Genetics and Genomic Sciences, Mount Sinai School of Medicine, 1425 Madison Avenue, Box 1498, 10029, New York, NY, USA aut Lau Jennie Institute for Conservation Research, San Diego Zoo Global, 15600 San Pasqual Valley Road, 92027, Escondido, CA, USA aut Chemnick Leona Institute for Conservation Research, San Diego Zoo Global, 15600 San Pasqual Valley Road, 92027, Escondido, CA, USA aut Thompson Elizabeth Department of Statistics, University of Washington, Box 354322, 98195, Seattle, WA, USA aut Alberts Allison Institute for Conservation Research, San Diego Zoo Global, 15600 San Pasqual Valley Road, 92027, Escondido, CA, USA aut Ryder Oliver Institute for Conservation Research, San Diego Zoo Global, 15600 San Pasqual Valley Road, 92027, Escondido, CA, USA aut Gerber Glenn Institute for Conservation Research, San Diego Zoo Global, 15600 San Pasqual Valley Road, 92027, Escondido, CA, USA aut Conservation Genetics Springer Netherlands 12/3(2011-06-01), 771-781 1566-0621 12:3<771 2011 12 10592 https://doi.org/10.1007/s10592-011-0184-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10592-011-0184-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mitchell Adele Department of Genetics and Genomic Sciences, Mount Sinai School of Medicine, 1425 Madison Avenue, Box 1498, 10029, New York, NY, USA aut NATIONALLICENCE 700 1- Lau Jennie Institute for Conservation Research, San Diego Zoo Global, 15600 San Pasqual Valley Road, 92027, Escondido, CA, USA aut NATIONALLICENCE 700 1- Chemnick Leona Institute for Conservation Research, San Diego Zoo Global, 15600 San Pasqual Valley Road, 92027, Escondido, CA, USA aut NATIONALLICENCE 700 1- Thompson Elizabeth Department of Statistics, University of Washington, Box 354322, 98195, Seattle, WA, USA aut NATIONALLICENCE 700 1- Alberts Allison Institute for Conservation Research, San Diego Zoo Global, 15600 San Pasqual Valley Road, 92027, Escondido, CA, USA aut NATIONALLICENCE 700 1- Ryder Oliver Institute for Conservation Research, San Diego Zoo Global, 15600 San Pasqual Valley Road, 92027, Escondido, CA, USA aut NATIONALLICENCE 700 1- Gerber Glenn Institute for Conservation Research, San Diego Zoo Global, 15600 San Pasqual Valley Road, 92027, Escondido, CA, USA aut NATIONALLICENCE 773 0- Conservation Genetics Springer Netherlands 12/3(2011-06-01), 771-781 1566-0621 12:3<771 2011 12 10592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer