caa a22 4500 445857633 CHVBK 20180317145433.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10592-011-0198-9 doi (NATIONALLICENCE)springer-10.1007/s10592-011-0198-9 Patterns of chloroplast DNA variation in Cycas debaoensis (Cycadaceae): conservation implications [Elektronische Daten] [Qing-Qing Zhan, Jin-Feng Wang, Xun Gong, Hua Peng] Climate changes during glacial periods have had significant effects on the current distribution of plant species. Palaeontologial data suggest that modern cycads originated in southwest China. Cycas debaoensis (Cycadaceae) is an endangered species restricted to a small area of southwest China. This species has been classified into two types: sand and karst, according to the soil matrix they grow on. To determine the locations of its glacial refugia and its genetic structure, we examined chloroplast sequence variation of the atpB-rbcL and psbA-trnH intergenic spacers. Four chloroplast DNA haplotypes were obtained from 120 individuals collected from 11 populations covering the entire extant distribution range of the species. Significant population subdivision was detected (G ST=0.684 and F ST=0.74160), suggesting low levels of gene flow between regions and populations. There was marked haplotype differentiation between populations in the sand and karst regions, with only one haplotype being present in both. The molecular phylogenetic data, together with the geographic distribution of the haplotypes, suggest that C. debaoensis experienced range contraction during glacial periods, and that the current populations are still confined to the areas of the original refugia. These results implied that isolated refugia might have maintained in both sand and karst regions during the last glacial maximum and even earlier glaciations. The low within-population diversity of C. debaoensis suggested that there were strong bottleneck events or founder effects within each separate region during the Quaternary climatic oscillations. These findings are important for the conservation of this endangered species. Springer Science+Business Media B.V., 2011 Chloroplast DNA atp B- rbc L and psb A- trn H nationallicence Cycas debaoensis nationallicence Glacial refugia nationallicence Range contraction nationallicence Population structure nationallicence Conservation nationallicence Zhan Qing-Qing Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, 650204, Kunming, People's Republic of China aut Wang Jin-Feng Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, 650204, Kunming, People's Republic of China aut Gong Xun Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, 650204, Kunming, People's Republic of China aut Peng Hua Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, 650204, Kunming, People's Republic of China aut Conservation Genetics Springer Netherlands 12/4(2011-08-01), 959-970 1566-0621 12:4<959 2011 12 10592 https://doi.org/10.1007/s10592-011-0198-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10592-011-0198-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhan Qing-Qing Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, 650204, Kunming, People's Republic of China aut NATIONALLICENCE 700 1- Wang Jin-Feng Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, 650204, Kunming, People's Republic of China aut NATIONALLICENCE 700 1- Gong Xun Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, 650204, Kunming, People's Republic of China aut NATIONALLICENCE 700 1- Peng Hua Key Laboratory of Biodiversity and Biogeography, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, 650204, Kunming, People's Republic of China aut NATIONALLICENCE 773 0- Conservation Genetics Springer Netherlands 12/4(2011-08-01), 959-970 1566-0621 12:4<959 2011 12 10592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer