caa a22 4500 445857730 CHVBK 20180317145433.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10592-011-0195-z doi (NATIONALLICENCE)springer-10.1007/s10592-011-0195-z High connectivity among argali sheep from Afghanistan and adjacent countries: Inferences from neutral and candidate gene microsatellites [Elektronische Daten] [G. Luikart, S. Amish, J. Winnie, A. Beja-Pereira, R. Godinho, F. Allendorf, R. Harris] We quantified population connectivity and genetic variation in the Marco Polo subspecies of argali mountain sheep (Ovis ammon polii) by genotyping 9 neutral and 8 candidate gene microsatellite loci in 172 individuals noninvasively sampled across five study areas in Afghanistan, China, and Tajikistan. Heterozygosity and allelic richness were generally high (mean H=0.67, mean A=6.1), but were significantly lower in the China study area (H=0.61, P<0.001; A=4.9, P<0.01). One marker in an immune system gene (TCRG4) showed an excess of rare alleles compared to neutral expectations. Another immune system gene (GLYCAM-1) showed excessive differentiation (high F ST) between study areas. Estimates of genetic differentiation were similar (F ST=0.035 vs. 0.033) with and without the two loci deviating from neutrality, suggesting that selection is not a primary driver of overall molecular variation, and that candidate gene loci can be used for connectivity monitoring, as long as selection tests are conducted to avoid biased gene flow estimates. Adequate protection of argali and maintenance of inter-population connectivity will require monitoring and international cooperation because argali exhibit high gene flow across international borders. Springer Science+Business Media B.V., 2011 Bottlenecks nationallicence Habitat fragmentation nationallicence Gene flow nationallicence Ovis ammon nationallicence Pamir Mountains nationallicence Natural selection nationallicence Adaptation nationallicence Infectious disease nationallicence Noninvasive genetic monitoring nationallicence Mountain ungulate nationallicence Luikart G. Fish and Wildlife Genomics Group, Flathead Lake Biological Station, University of Montana, 59860, Polson, MT, USA aut Amish S. Fish and Wildlife Genomics Group, Division of Biological Sciences, University of Montana, 59812, Missoula, MT, USA aut Winnie J. Department of Ecosystem and Conservation Science, University of Montana, 59812, Missoula, MT, USA aut Beja-Pereira A. CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, 4485-661, Vairão, Portugal aut Godinho R. CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, 4485-661, Vairão, Portugal aut Allendorf F. Fish and Wildlife Genomics Group, Division of Biological Sciences, University of Montana, 59812, Missoula, MT, USA aut Harris R. Department of Ecosystem and Conservation Science, University of Montana, 59812, Missoula, MT, USA aut Conservation Genetics Springer Netherlands 12/4(2011-08-01), 921-931 1566-0621 12:4<921 2011 12 10592 https://doi.org/10.1007/s10592-011-0195-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10592-011-0195-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Luikart G. Fish and Wildlife Genomics Group, Flathead Lake Biological Station, University of Montana, 59860, Polson, MT, USA aut NATIONALLICENCE 700 1- Amish S. Fish and Wildlife Genomics Group, Division of Biological Sciences, University of Montana, 59812, Missoula, MT, USA aut NATIONALLICENCE 700 1- Winnie J. Department of Ecosystem and Conservation Science, University of Montana, 59812, Missoula, MT, USA aut NATIONALLICENCE 700 1- Beja-Pereira A. CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, 4485-661, Vairão, Portugal aut NATIONALLICENCE 700 1- Godinho R. CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Campus Agrário de Vairão, 4485-661, Vairão, Portugal aut NATIONALLICENCE 700 1- Allendorf F. Fish and Wildlife Genomics Group, Division of Biological Sciences, University of Montana, 59812, Missoula, MT, USA aut NATIONALLICENCE 700 1- Harris R. Department of Ecosystem and Conservation Science, University of Montana, 59812, Missoula, MT, USA aut NATIONALLICENCE 773 0- Conservation Genetics Springer Netherlands 12/4(2011-08-01), 921-931 1566-0621 12:4<921 2011 12 10592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer