caa a22 4500 445857749 CHVBK 20180317145433.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10592-011-0204-2 doi (NATIONALLICENCE)springer-10.1007/s10592-011-0204-2 Patterns of differentiation among endangered pondberry populations [Elektronische Daten] [Craig Echt, Dennis Demeer, Danny Gustafson] Pondberry, Lindera melissifolia, is an endangered and partially clonally reproducing shrub species found in isolated populations that inhabit seasonally wet depressions in forested areas of the lower Mississippi River alluvial valley and southeastern regions of the United States. With eleven microsatellite loci, we quantified population genetic differentiation and diversity among 450 genets in 10 locations distributed across pondberry's range. We used estimates of F st and Jost's D est to measure genetic differences between populations and between geographic regions. The largest pairwise regional difference was found between eastern and western regional population groups (F st=0.23, D est=0.67), with the northern-most population groups in each region exhibiting larger divergence from each other than the southern-most population groups. Genetic diversity was lowest in the Sand Pond Conservation Area (A e=1.9, H e=0.36), which was the northern-most pondberry population, and highest in the Francis Marion National Forest (A e=4.1, H e=0.69), although we identified only 17 genets in that admixed population. Following adjustments for estimated null allele frequencies, we identified heterozygote excess in four eastern populations and found no evidence for inbreeding in any population. The observed patterns of differentiation indicate a phylogeography that exhibits an Appalachian Mountain discontinuity coupled with northward migrations along the Southern Atlantic Coastal Plain and into the Mississippi Alluvial Plain. The genetic consequences of this proposed phylogeographical structure may affect selection of germplasm sources for population reestablishment programs across pondberry's range. Springer Science+Business Media B.V. (outside the USA), 2011 Clonal plant nationallicence Heterozygote excess nationallicence Phylogeography nationallicence Nuclear SSR markers nationallicence Echt Craig Southern Institute of Forest Genetics, Southern Research Station, USDA Forest Service, 23332 Success Road, 39574, Saucier, MS, USA aut Demeer Dennis Southern Institute of Forest Genetics, Southern Research Station, USDA Forest Service, 23332 Success Road, 39574, Saucier, MS, USA aut Gustafson Danny Department of Biology, The Citadel, 171 Moultrie Street, 29409, Charleston, SC, USA aut Conservation Genetics Springer Netherlands 12/4(2011-08-01), 1015-1026 1566-0621 12:4<1015 2011 12 10592 https://doi.org/10.1007/s10592-011-0204-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10592-011-0204-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Echt Craig Southern Institute of Forest Genetics, Southern Research Station, USDA Forest Service, 23332 Success Road, 39574, Saucier, MS, USA aut NATIONALLICENCE 700 1- Demeer Dennis Southern Institute of Forest Genetics, Southern Research Station, USDA Forest Service, 23332 Success Road, 39574, Saucier, MS, USA aut NATIONALLICENCE 700 1- Gustafson Danny Department of Biology, The Citadel, 171 Moultrie Street, 29409, Charleston, SC, USA aut NATIONALLICENCE 773 0- Conservation Genetics Springer Netherlands 12/4(2011-08-01), 1015-1026 1566-0621 12:4<1015 2011 12 10592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer