caa a22 4500 44585782X CHVBK 20180317145433.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10592-011-0212-2 doi (NATIONALLICENCE)springer-10.1007/s10592-011-0212-2 Paternal genotype reconstruction reveals multiple paternity and sex ratios in a breeding population of leatherback turtles ( Dermochelys coriacea ) [Elektronische Daten] [Kelly Stewart, Peter Dutton] When animals are difficult to observe while breeding, insights into the mating system may be gained by using molecular techniques. Patterns of extra-pair copulation, multiple paternity and parental genotype analysis may elucidate population characteristics that help improve knowledge of life history while informing management decisions. During the course of a long-term study of leatherback turtles, we assessed the level of multiple paternity in successive clutches for 12 known females nesting at Sandy Point National Wildlife Refuge (St. Croix, U.S. Virgin Islands). We used seven polymorphic microsatellite markers to genotype the females and 1,019 hatchlings representing 38 nests (3-4 clutches from each female). Using deductive genotype reconstruction and GERUD1.0, we identified the 12 mothers and 17 different fathers that were responsible for 38 nests. We found that seven females (58.3%) showed no evidence of multiple paternity in their clutches, while five females (41.7%) had mated with two males each. There was evidence of two fathers (polyandry) in successive clutches for these five females. Multiple fathers didn't contribute to clutches equally. For clutches laid by an individual female, the primary father was responsible for 53.7 to 85.9% of the hatchlings. We demonstrate the feasibility of using male genotype reconstruction to characterize the male component of this breeding population and to assess operational sex ratios for breeding sea turtles. US Government, 2011 Leatherback nationallicence Multiple paternity nationallicence St. Croix nationallicence Mating nationallicence Hatchlings nationallicence Sea turtle nationallicence Stewart Kelly Protected Resources Division, Southwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, 3333N. Torrey Pines Ct., 92037, La Jolla, CA, USA aut Dutton Peter Protected Resources Division, Southwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, 3333N. Torrey Pines Ct., 92037, La Jolla, CA, USA aut Conservation Genetics Springer Netherlands 12/4(2011-08-01), 1101-1113 1566-0621 12:4<1101 2011 12 10592 https://doi.org/10.1007/s10592-011-0212-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10592-011-0212-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stewart Kelly Protected Resources Division, Southwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, 3333N. Torrey Pines Ct., 92037, La Jolla, CA, USA aut NATIONALLICENCE 700 1- Dutton Peter Protected Resources Division, Southwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, 3333N. Torrey Pines Ct., 92037, La Jolla, CA, USA aut NATIONALLICENCE 773 0- Conservation Genetics Springer Netherlands 12/4(2011-08-01), 1101-1113 1566-0621 12:4<1101 2011 12 10592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer