caa a22 4500 445857846 CHVBK 20180317145433.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10592-011-0232-y doi (NATIONALLICENCE)springer-10.1007/s10592-011-0232-y Polyandry and multiple paternities in the threatened Agassiz's desert tortoise, Gopherus agassizii [Elektronische Daten] [Christina Davy, Taylor Edwards, Amy Lathrop, Mark Bratton, Mark Hagan, Brian Henen, Kenneth Nagy, Jonathon Stone, L. Hillard, Robert Murphy] We used data from 17 to 20 microsatellite markers to investigate the incidence of multiple paternities in wild Agassiz's desert tortoises, Gopherus agassizii. Neonates were sampled from clutches of eggs laid by wild mothers in nesting enclosures at Edwards Air Force Base and at the Marine Corps Air Ground Combat Center, California. We genotyped 28 clutches from 26 females sampling an average of six neonates per clutch. The number of paternal alleles was used to determine the minimum number of sires for each clutch. Based on conservative criteria requiring evidence from at least two loci to determine multiple paternity, a minimum of 64% of females were polyandrous, while a minimum of 57% of clutches were sired by multiple males. This formed one of the highest incidences of multiple paternities recorded to date in any species of tortoise. The high number of microsatellite loci involved in the analyses allowed detection of multiple paternities in clutches where this may have been missed if fewer loci were used. Our results highlighted the potential pitfalls of quantitatively comparing paternity studies based on differing sampling strategies. Finally, we summarized the conservation implications of the high rate of multiple paternities in this threatened species. Springer Science+Business Media B.V., 2011 CERVUS nationallicence Chelonids nationallicence Microsatellites nationallicence Reproductive strategy nationallicence Tortoise conservation nationallicence Davy Christina Department of Ecology and Evolutionary Biology, University of Toronto, 25 Wilcocks St., M5S 3B2, Toronto, ON, Canada aut Edwards Taylor Arizona Research Laboratories, University of Arizona Genetics Core, University of Arizona, 1657 E. Helen Street, 85721, Tucson, AZ, USA aut Lathrop Amy Department of Natural History, Royal Ontario Museum, 100 Queen's Park, M5S 2C6, Toronto, ON, Canada aut Bratton Mark JT3/CH2MHIll, Natural Resources Department, 5 East Popson Avenue, Building 2650A, 93524-8060, Edwards Air Force Base, CA, USA aut Hagan Mark USAF, Environmental Management Office, 5 East Popson Avenue, Building 2650A, 93524-8060, Edwards Air Force Base, CA, USA aut Henen Brian Natural Resources and Environmental Affairs, MACGFTC MCAGCC, 92278, Twentynine Palms, CA, USA aut Nagy Kenneth Department of Ecology and Evolutionary Biology, University of California, 621 Young Drive, 90095-1606, Los Angeles, CA, USA aut Stone Jonathon Department of Biology, McMaster University, 1280 Main Street West, L8S 4K1, Hamilton, ON, Canada aut Hillard L. Department of Ecology and Evolutionary Biology, University of California, 621 Young Drive, 90095-1606, Los Angeles, CA, USA aut Murphy Robert Department of Ecology and Evolutionary Biology, University of Toronto, 25 Wilcocks St., M5S 3B2, Toronto, ON, Canada aut Conservation Genetics Springer Netherlands 12/5(2011-10-01), 1313-1322 1566-0621 12:5<1313 2011 12 10592 https://doi.org/10.1007/s10592-011-0232-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10592-011-0232-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Davy Christina Department of Ecology and Evolutionary Biology, University of Toronto, 25 Wilcocks St., M5S 3B2, Toronto, ON, Canada aut NATIONALLICENCE 700 1- Edwards Taylor Arizona Research Laboratories, University of Arizona Genetics Core, University of Arizona, 1657 E. Helen Street, 85721, Tucson, AZ, USA aut NATIONALLICENCE 700 1- Lathrop Amy Department of Natural History, Royal Ontario Museum, 100 Queen's Park, M5S 2C6, Toronto, ON, Canada aut NATIONALLICENCE 700 1- Bratton Mark JT3/CH2MHIll, Natural Resources Department, 5 East Popson Avenue, Building 2650A, 93524-8060, Edwards Air Force Base, CA, USA aut NATIONALLICENCE 700 1- Hagan Mark USAF, Environmental Management Office, 5 East Popson Avenue, Building 2650A, 93524-8060, Edwards Air Force Base, CA, USA aut NATIONALLICENCE 700 1- Henen Brian Natural Resources and Environmental Affairs, MACGFTC MCAGCC, 92278, Twentynine Palms, CA, USA aut NATIONALLICENCE 700 1- Nagy Kenneth Department of Ecology and Evolutionary Biology, University of California, 621 Young Drive, 90095-1606, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Stone Jonathon Department of Biology, McMaster University, 1280 Main Street West, L8S 4K1, Hamilton, ON, Canada aut NATIONALLICENCE 700 1- Hillard L. Department of Ecology and Evolutionary Biology, University of California, 621 Young Drive, 90095-1606, Los Angeles, CA, USA aut NATIONALLICENCE 700 1- Murphy Robert Department of Ecology and Evolutionary Biology, University of Toronto, 25 Wilcocks St., M5S 3B2, Toronto, ON, Canada aut NATIONALLICENCE 773 0- Conservation Genetics Springer Netherlands 12/5(2011-10-01), 1313-1322 1566-0621 12:5<1313 2011 12 10592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer