caa a22 4500 445857951 CHVBK 20180317145434.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10592-011-0229-6 doi (NATIONALLICENCE)springer-10.1007/s10592-011-0229-6 Resurrecting an extinct species: archival DNA, taxonomy, and conservation of the Vegas Valley leopard frog [Elektronische Daten] [Evon Hekkala, Raymond Saumure, Jef Jaeger, Hans-Werner Herrmann, Michael Sredl, David Bradford, Danielle Drabeck, Michael Blum] Suggestions that the extinct Vegas Valley leopard frog (Rana fisheri=Lithobates fisheri) may have been synonymous with one of several declining species have complicated recovery planning for imperiled leopard frogs in southwestern United States. To address this concern, we reconstructed the phylogenetic position of R. fisheri from mitochondrial and nuclear sequence data obtained from century-old museum specimens. Analyses incorporating representative North American Rana species placed archival specimens within the clade comprising federally Threatened Chiricahua leopard frogs (Rana chiricahuensis=Lithobates chiricahuensis). Further analysis of Chiricahua leopard frogs recovered two diagnosable lineages. One lineage is composed of R. fisheri specimens and R. chiricahuensis near the Mogollon Rim in central Arizona, while the other encompasses R. chiricahuensis populations to the south and east. These findings ascribe R. chiricahuensis populations from the northwestern most portion of its range to a resurrected R. fisheri, demonstrating how phylogenetic placement of archival specimens can inform recovery and conservation plans, especially those that call for translocation, re-introduction, or population augmentation of imperiled species. The Author(s), 2011 Archival DNA nationallicence Museum specimens nationallicence Rana fisheri nationallicence Rana chiricahuensis nationallicence Taxonomy nationallicence Conservation genetics nationallicence Hekkala Evon Fordham University, 10458, New York, USA aut Saumure Raymond Research Division, Las Vegas Springs Preserve, 89107, Las Vegas, NV, USA aut Jaeger Jef School of Life Sciences and Public Lands Institute, University of Nevada, 89154-4004, Las Vegas, NV, USA aut Herrmann Hans-Werner School of Natural Resources and Environment and Arizona Cooperative Fish and Wildlife Research Unit (USGS), University of Arizona, 85721, Tucson, AZ, USA aut Sredl Michael Arizona Game and Fish Department, Nongame Branch, 85086, Phoenix, AZ, USA aut Bradford David US Environmental Protection Agency, National Exposure Research Lab, 89193-3478, Las Vegas, NV, USA aut Drabeck Danielle Department of Ecology and Evolutionary Biology, Tulane University, 70118, New Orleans, LA, USA aut Blum Michael Department of Ecology and Evolutionary Biology, Tulane University, 70118, New Orleans, LA, USA aut Conservation Genetics Springer Netherlands 12/5(2011-10-01), 1379-1385 1566-0621 12:5<1379 2011 12 10592 https://doi.org/10.1007/s10592-011-0229-6 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10592-011-0229-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hekkala Evon Fordham University, 10458, New York, USA aut NATIONALLICENCE 700 1- Saumure Raymond Research Division, Las Vegas Springs Preserve, 89107, Las Vegas, NV, USA aut NATIONALLICENCE 700 1- Jaeger Jef School of Life Sciences and Public Lands Institute, University of Nevada, 89154-4004, Las Vegas, NV, USA aut NATIONALLICENCE 700 1- Herrmann Hans-Werner School of Natural Resources and Environment and Arizona Cooperative Fish and Wildlife Research Unit (USGS), University of Arizona, 85721, Tucson, AZ, USA aut NATIONALLICENCE 700 1- Sredl Michael Arizona Game and Fish Department, Nongame Branch, 85086, Phoenix, AZ, USA aut NATIONALLICENCE 700 1- Bradford David US Environmental Protection Agency, National Exposure Research Lab, 89193-3478, Las Vegas, NV, USA aut NATIONALLICENCE 700 1- Drabeck Danielle Department of Ecology and Evolutionary Biology, Tulane University, 70118, New Orleans, LA, USA aut NATIONALLICENCE 700 1- Blum Michael Department of Ecology and Evolutionary Biology, Tulane University, 70118, New Orleans, LA, USA aut NATIONALLICENCE 773 0- Conservation Genetics Springer Netherlands 12/5(2011-10-01), 1379-1385 1566-0621 12:5<1379 2011 12 10592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer