caa a22 4500 445857994 CHVBK 20180317145434.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10592-011-0217-x doi (NATIONALLICENCE)springer-10.1007/s10592-011-0217-x Patterns of variability at the major histocompatibility class I and class II loci in populations of the endangered cyprinid Ladigesocypris ghigii [Elektronische Daten] [Katerina Moutou, Zissis Mamuris, Tania Firme, Maria Kontou, Theologia Sarafidou, Maria Stoumboudi] The patterns of MHC diversity were studied at UAA and DAB1 loci and the two domains involved in the recognition of antigenic peptides (α2 and β1, respectively) in eight Ladigesocypris ghigii populations inhabiting streams and a concrete reservoir, in order to understand the significance of these genes in bottlenecked populations of an endemic species and develop conservation rationale. In agreement with previous study employing RAPD and mtDNA markers (Mamuris et al., Freshw Biol 50:1441-1453, 2005), both loci exhibited a very low level of polymorphism with only two and four alleles detected for UAA and DAB1, respectively. The functional MHC diversity was even lower since UAA alleles were distinguished by a single synonymous substitution. The type of habitat did not affect the level of polymorphism. Our data suggest that DAB1 polymorphism might be the outcome of the positive selection, imposed by the temporal and spatial variation of pathogen load, and the genetic drift as a result of successive habitat shrinkage and deterioration by water abstraction year after year. The populations studied were significantly less diverged at MHC loci than expected based on nuclear and mtDNA markers, suggesting that common parasites might act as causative factors to homogenize selection. Sufficient epidemiological data are required for the interpretation of the results and decision-making on suitable conservation actions. Springer Science+Business Media B.V., 2011 Ladigesocypris ghigii nationallicence MHC diversity nationallicence UAA nationallicence DAB1 nationallicence Conservation nationallicence Moutou Katerina Department of Biochemistry and Biotechnology, University of Thessaly, 26 Ploutonos Street, 412 21, Larissa, Greece aut Mamuris Zissis Department of Biochemistry and Biotechnology, University of Thessaly, 26 Ploutonos Street, 412 21, Larissa, Greece aut Firme Tania Department of Biochemistry and Biotechnology, University of Thessaly, 26 Ploutonos Street, 412 21, Larissa, Greece aut Kontou Maria Department of Biochemistry and Biotechnology, University of Thessaly, 26 Ploutonos Street, 412 21, Larissa, Greece aut Sarafidou Theologia Department of Biochemistry and Biotechnology, University of Thessaly, 26 Ploutonos Street, 412 21, Larissa, Greece aut Stoumboudi Maria Institute of Inland Waters, Hellenic Centre for Marine Research, P.O. Box 712, 190 13, Anavyssos, Greece aut Conservation Genetics Springer Netherlands 12/5(2011-10-01), 1159-1171 1566-0621 12:5<1159 2011 12 10592 https://doi.org/10.1007/s10592-011-0217-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10592-011-0217-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moutou Katerina Department of Biochemistry and Biotechnology, University of Thessaly, 26 Ploutonos Street, 412 21, Larissa, Greece aut NATIONALLICENCE 700 1- Mamuris Zissis Department of Biochemistry and Biotechnology, University of Thessaly, 26 Ploutonos Street, 412 21, Larissa, Greece aut NATIONALLICENCE 700 1- Firme Tania Department of Biochemistry and Biotechnology, University of Thessaly, 26 Ploutonos Street, 412 21, Larissa, Greece aut NATIONALLICENCE 700 1- Kontou Maria Department of Biochemistry and Biotechnology, University of Thessaly, 26 Ploutonos Street, 412 21, Larissa, Greece aut NATIONALLICENCE 700 1- Sarafidou Theologia Department of Biochemistry and Biotechnology, University of Thessaly, 26 Ploutonos Street, 412 21, Larissa, Greece aut NATIONALLICENCE 700 1- Stoumboudi Maria Institute of Inland Waters, Hellenic Centre for Marine Research, P.O. Box 712, 190 13, Anavyssos, Greece aut NATIONALLICENCE 773 0- Conservation Genetics Springer Netherlands 12/5(2011-10-01), 1159-1171 1566-0621 12:5<1159 2011 12 10592 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer